US 12,139,614 B2
Photoactivatable fluorescent dyes with hydrophilic caging groups and their use
Michael Weber, Goettingen (DE); Stefan W. Hell, Goettingen (DE); and Alexey Butkevich, Goettingen (DE)
Assigned to Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e. V., Munich (DE)
Filed by Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e. V., Munich (DE)
Filed on Aug. 24, 2021, as Appl. No. 17/410,879.
Claims priority of application No. 20193361 (EP), filed on Aug. 28, 2020.
Prior Publication US 2022/0064452 A1, Mar. 3, 2022
Int. Cl. C09B 11/24 (2006.01); C12Q 1/6818 (2018.01)
CPC C09B 11/24 (2013.01) [C12Q 1/6818 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A compound which is a photoactivable fluorescent dye having the structural formula I:

OG Complex Work Unit Chemistry
wherein:
n=0, 1, 2, 3;
X is selected from O, CRR′, SiRR′ and GeRR′, where R and R′ represent independently alkyl, cycloalkyl, alkenyl, alkynyl or aryl;
Y is H, SO3H or SO3M, with M being a positively charged counterion;
R1 is H, CO2H, C(O)NH-linker-CO2H, C(O)O-ligand, C(O)NH-ligand or C(O)NH-linker-ligand;
R2 is H, unsubstituted alkyl, substituted alkyl, unsubstituted cycloalkyl or substituted cycloalkyl;
R3 and R4 are independently H or F;
R5 is H, Me, CO2H, C(O)NH-linker-CO2H, C(O)O-ligand, C(O)NH-ligand or C(O)NH-linker-ligand;
wherein the ligand at each occurrence represents a reactive group or tag, capable to form a covalent or non-covalent bond or molecular complex with a target chemical entity or substance;
and wherein

OG Complex Work Unit Chemistry