US 12,139,519 B2
CRY2 variant having increased photosensitivity and use thereof
Wondo Heo, Daejeon (KR); Sungsoo Kim, Jinju-si (KR); Sang Kyu Lee, Seoul (KR); and Taeyoon Kyung, Incheon (KR)
Assigned to INSTITUTE FOR BASIC SCIENCE, Daejeon (KR)
Appl. No. 17/293,568
Filed by INSTITUTE FOR BASIC SCIENCE, Daejeon (KR)
PCT Filed Nov. 13, 2019, PCT No. PCT/KR2019/015459
§ 371(c)(1), (2) Date May 13, 2021,
PCT Pub. No. WO2020/101355, PCT Pub. Date May 22, 2020.
Claims priority of application No. 10-2018-0139283 (KR), filed on Nov. 13, 2018.
Prior Publication US 2022/0002362 A1, Jan. 6, 2022
Int. Cl. C07K 14/47 (2006.01); C07K 14/705 (2006.01); C12N 15/86 (2006.01)
CPC C07K 14/4703 (2013.01) [C07K 14/705 (2013.01); C12N 15/86 (2013.01); C12N 2800/107 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A CRY2 variant in which
at least one of E279 and E281 having a negative charge among a N277SEGE281 sequence of SEQ ID NO: 1 is substituted with any one selected from the neutral amino acid group of alanine (A), isoleucine (I), leucine (L), valine (V), phenylalanine (F), methionine (M), and tryptophan (W), or
S278 or G280 in the N277SEGE281 sequence of SEQ ID NO: 1 is substituted with any one selected from a bulky amino acid group of tryptophan (W) and phenylalanine (F).