	US 12,139,516 B2
	Purification of phycobiliproteins
Olivier Cagnac, Libourne (FR); Axel Athane, Carbon-Blanc (FR); and Julien Demol, Les Billaux (FR)
Assigned to FERMENTALG, Libourne (FR)
Appl. No. 16/497,510
Filed by FERMENTALG, Libourne (FR)
PCT Filed Mar. 30, 2018, PCT No. PCT/EP2018/058294 § 371(c)(1), (2) Date Sep. 25, 2019, PCT Pub. No. WO2018/178334, PCT Pub. Date Oct. 4, 2018.
Claims priority of application No. 1752674 (FR), filed on Mar. 30, 2017.
Prior Publication US 2020/0115423 A1, Apr. 16, 2020
Int. Cl. C07K 14/405 (2006.01); A23L 5/46 (2016.01); C07K 1/12 (2006.01); C07K 1/14 (2006.01); C12N 1/12 (2006.01)

CPC C07K 14/405 (2013.01) [A23L 5/46 (2016.08); C07K 1/145 (2013.01); C12N 1/12 (2013.01)]

6 Claims

1. A method for purifying acid-pH-resistant phycocyanins from a crude extract of acid-pH-resistant phycocyanins from cells of a phycocyanin-producing microorganism, said microorganism being of the genus Galdieria, said acid-pH-resistant phycocyanins being phycocyanins stable at a pH less than 5, and

wherein the method comprises the steps of:

a) adjusting the pH of the crude extract of acid-pH-resistant phycocyanins to a pH below 5 so as to precipitate organic matter other than acid-pH-resistant phycocyanins,

b) recovering the supernatant comprising acid-pH-resistant phycocyanins obtained in step a) and

c) isolating acid-pH-resistant phycocyanins from the supernatant of step b).