	US 12,139,511 B2
	Influenza virus hemagglutinin mutants
Pierre-Olivier Lavoie, Quebec (CA); Aurelien Lorin, Quebec (CA); Alain Doucet, Quebec (CA); Marc-Andre D'Aoust, Quebec (CA); and Manon Couture, Quebec (CA)
Assigned to Aramis Biotechnologies INC., Quebec (CA)
Appl. No. 17/255,520
Filed by MEDICAGO INC., Quebec (CA)
PCT Filed Jun. 27, 2019, PCT No. PCT/CA2019/050892 § 371(c)(1), (2) Date Dec. 23, 2020, PCT Pub. No. WO2020/000100, PCT Pub. Date Jan. 2, 2020.
Claims priority of provisional application 62/690,780, filed on Jun. 27, 2018.
Prior Publication US 2021/0371472 A1, Dec. 2, 2021
Int. Cl. C07K 14/11 (2006.01); A61K 38/00 (2006.01); A61K 39/145 (2006.01); A61P 31/16 (2006.01); A61P 37/04 (2006.01); C07K 14/005 (2006.01); C07K 16/10 (2006.01); C12N 7/00 (2006.01); C12N 15/82 (2006.01); A61K 39/00 (2006.01)

CPC C07K 14/005 (2013.01) [A61K 39/145 (2013.01); A61P 31/16 (2018.01); A61P 37/04 (2018.01); C07K 14/11 (2013.01); C07K 16/10 (2013.01); C07K 16/1018 (2013.01); C12N 7/00 (2013.01); C12N 15/8257 (2013.01); C12N 15/8258 (2013.01); A61K 38/00 (2013.01); A61K 2039/5258 (2013.01); C12N 2760/16023 (2013.01); C12N 2760/16033 (2013.01); C12N 2760/16122 (2013.01); C12N 2760/16123 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16152 (2013.01)]

4 Claims

1. A nucleic acid comprising a nucleotide sequence encoding a modified influenza H3 hemagglutinin (HA) protein, the HA protein comprising an amino acid sequence of a wildtype influenza H3 HA protein and comprising at least one amino acid substitution, said at least one amino acid substitution consisting of an amino acid substitution corresponding to position 382 of reference sequence SEQ ID NO: 92 and optionally one or more amino acid substitution corresponding to positions 384, 392 and/or 431 of reference sequence SEQ ID NO: 92,

wherein:

the amino acid substitution corresponding to position 382 of reference sequence SEQ ID NO: 92 is to an alanine or a conserved substitution of alanine;

the amino acid substitution corresponding to position 384 of reference sequence SEQ ID NO: 92 is to a valine or a conserved substitution of valine;

the amino acid substitution corresponding to position 392 of reference sequence SEQ ID NO: 92 is to an aspartic acid or a conserved substitution of aspartic acid; and

the amino acid substitution corresponding to position 431 of reference sequence SEQ ID NO: 92 is to a methionine or a conserved substitution of methionine.