US 12,139,510 B2
Process of purification of protein
Om Narayan, Ahmedabad (IN); Tarun Kumar Gupta, Ahmedabad (IN); and Mayankkumar Thakkar, Ahmedabad (IN)
Assigned to Kashiv BioSciences, LLC, Piscataway, NJ (US)
Filed by Kashiv BioSciences, LLC, Piscataway, NJ (US)
Filed on Jan. 18, 2024, as Appl. No. 18/416,844.
Application 18/416,844 is a continuation of application No. 17/922,729, previously published as PCT/IB2021/053658, filed on May 1, 2021.
Claims priority of application No. 202021018714 (IN), filed on May 1, 2020; application No. 202021018731 (IN), filed on May 1, 2020; and application No. 202021018735 (IN), filed on May 1, 2020.
Prior Publication US 2024/0239838 A1, Jul. 18, 2024
Int. Cl. C07K 1/16 (2006.01); C07K 16/06 (2006.01); C07K 16/42 (2006.01)
CPC C07K 1/16 (2013.01) [C07K 16/065 (2013.01); C07K 16/4291 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A process of purifying a protein of interest from a protein mixture comprising the protein of interest and one or more basic variants, the process comprising:
a) loading the protein mixture onto a ceramic hydroxyapatite column with a loading buffer;
b) washing the ceramic hydroxyapatite column with a washing buffer;
c) eluting by a linear gradient with an eluting buffer, thereby producing an eluted protein mixture comprising a purified protein of interest;
wherein the washing buffer is sodium phosphate buffer;
wherein the eluting buffer is sodium phosphate buffer; and
wherein the purified protein of interest is substantially pure and the one or more basic variants are present in the eluted protein mixture in an amount below 15% as analysed by cation exhange high performance liquid chromatography.