US 12,138,952 B2
Nanostructured surfaces
Thomas J. Webster, Barrington, RI (US)
Assigned to BROWN UNIVERSITY, Providence, RI (US)
Filed by Brown University, Providence, RI (US)
Filed on Dec. 23, 2022, as Appl. No. 18/146,158.
Application 18/146,158 is a continuation of application No. 16/692,744, filed on Nov. 22, 2019, granted, now 11,560,014.
Application 16/692,744 is a continuation of application No. 13/639,928, granted, now 10,493,793, issued on Dec. 3, 2019, previously published as PCT/US2011/031890, filed on Apr. 11, 2011.
Claims priority of provisional application 61/418,838, filed on Dec. 1, 2010.
Claims priority of provisional application 61/322,366, filed on Apr. 9, 2010.
Prior Publication US 2023/0311563 A1, Oct. 5, 2023
Int. Cl. B44C 1/22 (2006.01); A61M 16/04 (2006.01); C12N 1/36 (2006.01); C12N 11/00 (2006.01); C23F 1/14 (2006.01)
CPC B44C 1/227 (2013.01) [A61M 16/04 (2013.01); C12N 1/36 (2013.01); C12N 11/00 (2013.01); C23F 1/14 (2013.01); Y10T 428/24355 (2015.01)] 31 Claims
 
1. An anti-bacterial substrate comprising a substrate and a polymer substrate surface having a nanometer scale surface geometry sufficient to inhibit adherence of bacterial cells to the polymer substrate surface;
wherein the polymer substrate surface is selected from the group consisting of one or more of silicone, polyurethane, polycaprolactone, poly-lactic-co-glycolic acid, poly-lactic acid, poly-glycolic acid, polyethylene, polyethylene glycol, polydimethylsiloxane, polyacrylamide, polypropylene, polystyrene, polyether ether ketone (PEEK), ultra-high molecular weight polyethylene (UHMWPE), hydrogels, and composites thereof;
wherein the nanometer scale surface geometry includes features having nanometer scale dimensions, the features comprising one or more shapes of dots, spots, hills, points, mounds, valleys, slopes, or a combination thereof;
wherein the nanometer scale surface geometry is operative to inhibit a colonization of the bacterial cells on the polymer substrate surface as compared to the colonization of the bacterial cells that would be observed on the same polymer substrate surface but without the nanometer scale surface geometry;
wherein a colonization of the bacteria cells on the polymer substrate surface is inhibited by about 30% to about 50%, as measured by contacting the polymer substrate surface with Staphylococcus aureus bacteria for 4 to 72 hours as compared to contacting the same surface but without the nanometer scale surface geometry with Staphylococcus aureus bacteria for 4 to 72 hours.