US 12,138,626 B2
In situ-generated microfluidic isolation structures, kits and methods of use thereof
Kristin G. Beaumont, New York, NY (US); Nan-Linda Ding, Vancouver (CA); Volker L. S. Kurz, Oakland, CA (US); Troy A. Lionberger, Berkeley, CA (US); Randall D. Lowe, Jr., Emeryville, CA (US); Daniele Malleo, San Jose, CA (US); Andrew W. McFarland, Berkeley, CA (US); J. Tanner Nevill, El Cerrito, CA (US); and Xiaohua Wang, Albany, CA (US)
Assigned to BRUKER CELLULAR ANALYSIS, INC., Emeryville, CA (US)
Filed by BRUKER CELLULAR ANALYSIS, INC., Emeryville, CA (US)
Filed on Feb. 3, 2023, as Appl. No. 18/164,124.
Application 18/164,124 is a division of application No. 15/359,115, filed on Nov. 22, 2016, granted, now 11,666,913.
Claims priority of provisional application 62/423,627, filed on Nov. 17, 2016.
Claims priority of provisional application 62/258,957, filed on Nov. 23, 2015.
Prior Publication US 2023/0182136 A1, Jun. 15, 2023
Int. Cl. B01L 3/00 (2006.01); B01J 19/00 (2006.01); G01N 27/447 (2006.01)
CPC B01L 3/502753 (2013.01) [B01J 19/0093 (2013.01); B01L 3/5023 (2013.01); B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); G01N 27/44791 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/16 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0677 (2013.01); B01L 2400/08 (2013.01)] 20 Claims
 
1. A method of isolating a micro-object in a microfluidic device, comprising:
introducing the micro-object into an enclosure of the microfluidic device, the enclosure comprising a flow region and a sequestration pen comprising an isolation region and a connection region, the connection region having a proximal opening to the flow region and a distal opening to the isolation region;
disposing the micro-object into the sequestration pen;
introducing a solution comprising a flowable polymer into the enclosure;
solidifying the flowable polymer thereby forming an in situ-generated isolation structure, in-situ at least in part in the sequestration pen, wherein the in situ-generated isolation structure is disposed within the connection region and configured to block passage of the micro-object between the sequestration pen and the flow region; and
isolating the micro-object using the in situ-generated isolation structure.