US 12,138,366 B2
Magneto-patterned-cell-laden hydrogel materials and methods of making and using same
Hannah M. Zlotnick, Philadelphia, PA (US); Andrew Todd Clark, North Wales, PA (US); Robert L. Mauck, Philadelphia, PA (US); and Xuemei Cheng, Bryn Mawr, PA (US)
Assigned to The United States Government as Represented by the Department of Veterans Affairs, Washington, DC (US); and The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed by United States Government as represented by the Department of Veterans Affairs, Washington, DC (US); and The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed on Apr. 13, 2021, as Appl. No. 17/229,829.
Claims priority of provisional application 63/009,419, filed on Apr. 13, 2020.
Prior Publication US 2021/0316044 A1, Oct. 14, 2021
Int. Cl. A61L 27/38 (2006.01); A61L 27/04 (2006.01); A61L 27/22 (2006.01); A61L 27/46 (2006.01); A61L 27/52 (2006.01)
CPC A61L 27/3847 (2013.01) [A61L 27/042 (2013.01); A61L 27/045 (2013.01); A61L 27/047 (2013.01); A61L 27/222 (2013.01); A61L 27/225 (2013.01); A61L 27/3852 (2013.01); A61L 27/46 (2013.01); A61L 27/52 (2013.01); A61L 2300/258 (2013.01); A61L 2300/402 (2013.01); A61L 2300/406 (2013.01); A61L 2300/408 (2013.01); A61L 2300/41 (2013.01); A61L 2300/414 (2013.01); A61L 2300/426 (2013.01); A61L 2300/43 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of producing a continuous hydrogel material, the method comprising the steps of:
(a) providing a viscous mixture comprising a hydrogel precursor, a paramagnetic or superparamagnetic salt, magnetic objects comprising a magnetic material and an active agent, crosslinking agent, and cells in a mold, wherein the cells are (i) not labeled with any magnetic label, (ii) do not have any magnetic particles tethered to or within the cells, and (iii) are diamagnetic;
(b) creating a gradient of the cells in the viscous mixture through negative magnetophoresis by exposing the viscous mixture to a magnetic field, wherein exposing the viscous mixture to the magnetic field further creates a gradient of the magnetic objects in the viscous mixture that is opposite the gradient of the cells in the viscous mixture; and
(c) crosslinking the hydrogel precursor, thereby producing the continuous hydrogel material having a gradient of the cells that is opposite the gradient of the magnetic objects.