US 12,138,364 B2
Decellularised cell wall structures from fungus and use thereof as scaffold materials
Andrew Edward Pelling, Gloucester (CA); Charles Michel Cuerrier, Gatineau (CA); Daniel J. Modulevsky, Ottawa (CA); and Ryan Joseph Hickey, Ottawa (CA)
Assigned to University of Ottawa, Ottawa (CA)
Filed by University of Ottawa, Ottawa (CA)
Filed on Sep. 29, 2021, as Appl. No. 17/488,454.
Application 17/488,454 is a division of application No. 16/848,412, filed on Apr. 14, 2020, granted, now 11,167,062.
Application 16/848,412 is a division of application No. 16/076,990, granted, now 11,045,582, issued on Jun. 29, 2021, previously published as PCT/CA2017/050163, filed on Feb. 10, 2017.
Claims priority of provisional application 62/294,671, filed on Feb. 12, 2016.
Prior Publication US 2022/0016317 A1, Jan. 20, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61L 27/36 (2006.01); A61L 27/38 (2006.01); A61L 27/50 (2006.01); A61L 27/52 (2006.01); A61L 27/54 (2006.01); A61L 27/60 (2006.01); C12N 5/00 (2006.01)
CPC A61L 27/3637 (2013.01) [A61L 27/3683 (2013.01); A61L 27/3687 (2013.01); A61L 27/38 (2013.01); A61L 27/507 (2013.01); A61L 27/52 (2013.01); A61L 27/54 (2013.01); A61L 27/60 (2013.01); C12N 5/0062 (2013.01); C12N 5/0068 (2013.01); A61L 2300/414 (2013.01); A61L 2430/02 (2013.01); A61L 2430/16 (2013.01); A61L 2430/32 (2013.01); A61L 2430/40 (2013.01)] 11 Claims
 
1. A scaffold biomaterial comprising a decellularised fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularised fungal tissue comprising a chitin-based 3-dimensional porous structure,
wherein the decellularised fungal tissue has been decellularised by treatment with sodium dodecyl sulphate (SDS), and
wherein residual SDS has been removed by using an aqueous divalent salt solution to precipitate a salt residue containing SDS micelles out of the scaffold biomaterial.