US 12,138,352 B2
Nanohelix-substrate complex for controlling macrophage behavior, preparation method thereof, and method of controlling adhesion and polarization of macrophage by using the same
Young-Keun Kim, Seoul (KR); Heemin Kang, Seoul (KR); Min-Jun Ko, Seoul (KR); and Gunhyu Bae, Hwaseong-si (KR)
Assigned to Korea University Research and Business Foundation, Seoul (KR)
Filed by Korea University Research and Business Foundation, Seoul (KR)
Filed on Jul. 7, 2021, as Appl. No. 17/368,883.
Claims priority of application No. 10-2020-0131888 (KR), filed on Oct. 13, 2020.
Prior Publication US 2022/0110880 A1, Apr. 14, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/51 (2006.01); A61K 47/69 (2017.01); A61N 2/00 (2006.01); C12N 5/0786 (2010.01); C12N 13/00 (2006.01)
CPC A61K 9/5115 (2013.01) [A61K 9/5123 (2013.01); A61K 47/6929 (2017.08); A61N 2/00 (2013.01); C12N 5/0645 (2013.01); C12N 13/00 (2013.01); A61N 2/002 (2013.01)] 12 Claims
 
1. A metallic nanohelix-substrate complex for controlling adhesion and polarization of macrophages, the metallic nanohelix-substrate complex comprising:
a substrate;
one or more metallic nanohelices chemically coupled to the substrate; and
one or more integrin ligand peptides chemically coupled to the one or more metallic nanohelices,
wherein the one or more metallic nanohelices are formed of a spiral nanowire and comprise one or more metal elements selected from the group consisting of cobalt (Co), iron (Fe), and nickel (Ni),
the one or more metallic nanohelices have a length of 100 nm to 20 μm, and
the one or more metallic nanohelices have a length reversibly changed depending on application/non-application of a magnetic field within a range of Equation 1 below,
|L1−L0|>10 nm  [Equation 1]
in Equation 1, L1 is a length of the one or more metallic nanohelices when the magnetic field is applied, and L0 is a length of the one or more metallic nanohelices when the magnetic field is not applied,
wherein the one or more integrin ligand peptides comprise a thiolated integrin ligand peptide, and
wherein a thiol group of the thiolated integrin ligand peptide is coupled to the spiral nanowire by a polyethylene glycol linker.