US 12,138,348 B2
Dry powder composition comprising long-chain RNA
Fabian Johannes Eber, Stuttgart (DE); Benyamin Yazdan Panah, Tübingen (DE); Stefanie Sewing, Tübingen (DE); Thomas Ketterer, Gomaringen (DE); Thorsten Mutzke, Reutlingen (DE); Tilmann Roos, Kusterdingen (DE); Michael Sonntag, Tübingen (DE); Michael Wiggenhorn, Munich (DE); and Katharina Kolland, Augsburg (DE)
Assigned to CureVac SE, Tübingen (DE)
Filed by CureVac SE, Tübingen (DE)
Filed on Feb. 21, 2022, as Appl. No. 17/676,586.
Application 17/676,586 is a division of application No. 16/904,993, filed on Jun. 18, 2020.
Application 16/904,993 is a continuation of application No. 15/575,301, granted, now 10,729,654, issued on Aug. 4, 2020, previously published as PCT/EP2016/000843, filed on May 20, 2016.
Claims priority of application No. 15001517 (EP), filed on May 20, 2015; and application No. PCT/EP2015/002018 (WO), filed on Oct. 13, 2015.
Prior Publication US 2022/0168225 A1, Jun. 2, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); A61K 9/16 (2006.01); A61K 9/19 (2006.01); A61K 31/7105 (2006.01); A61K 48/00 (2006.01)
CPC A61K 9/1682 (2013.01) [A61K 9/19 (2013.01); A61K 31/7105 (2013.01); A61K 48/005 (2013.01); A61K 48/0091 (2013.01)] 20 Claims
 
1. A dry powder composition comprising long-chain RNA molecules having a length of 500 to 20,000 nucleotides, said composition produced by a method comprising:
a) providing a liquid comprising the long-chain RNA molecules, wherein the long-chain RNA molecules are in a complex with a cationic or polycationic compound,
b) drying the liquid provided of step a) by spray-freeze drying, wherein the spray-freeze drying comprises a step of atomization of the liquid at a temperature below −50° C., thereby producing the dry powder composition,
wherein the dry powder composition has a residual water content of 7% (w/w) or less,
wherein the dry powder composition comprises a plurality of particles having a mean diameter of about 100 μm to about 200 μm.