US 12,138,317 B1
Method of increasing cell adhesion with palygorskite
Sahel Nishat Abduljauwad, Dhahran (SA); and Habib-ur-Rehman Ahmed, Dhahran (SA)
Assigned to KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS, Dhahran (SA)
Filed by KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS, Dhahran (SA)
Filed on Jul. 19, 2024, as Appl. No. 18/777,706.
Application 18/777,706 is a continuation of application No. 15/877,601, filed on Jan. 23, 2018, granted, now 12,076,415.
Claims priority of provisional application 62/547,386, filed on Aug. 18, 2017.
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 47/69 (2017.01); A61K 9/51 (2006.01); A61K 33/26 (2006.01); A61K 35/02 (2015.01); A61K 35/28 (2015.01); A61K 47/02 (2006.01); A61P 35/04 (2006.01); C01B 33/38 (2006.01)
CPC A61K 47/6923 (2017.08) [A61K 9/5115 (2013.01); A61K 33/26 (2013.01); A61K 35/02 (2013.01); A61K 47/02 (2013.01); A61P 35/04 (2018.01); C01B 33/38 (2013.01); A61K 35/28 (2013.01)] 2 Claims
 
1. A method of increasing cell adhesion, comprising
preparing a composition by:
sonicating a mixture of nanosized smectite and saline to form the composition,
wherein the composition consists of saline and the nanosized smectite, and
contacting Raji cells with the composition,
wherein the nanosized smectite is palygorskite crystallites having a particle size of 30-75 nm and a cation exchange capacity (CEC) value of about 20 meq/100 g,
wherein the contacting results in an increase in adhesion force of the Raji cells to a target substrate of about 200%, compared to a same method but without contacting the Raji cells with the composition,
wherein the target substrate is an extracellular matrix (ECM) comprising fibronectin, and
wherein the adhesion force is measured between the fibronectin and the Raji cells by atomic force microscopy.