US 12,138,299 B2
Aldehyde dehydrogenase producing bacteria and methods of using same
Theresa Alenghat, Cincinnati, OH (US)
Assigned to Children's Hospital Medical Center, Cincinnati, OH (US)
Filed by Children's Hospital Medical Center, Cincinnati, OH (US)
Filed on Apr. 3, 2024, as Appl. No. 18/625,448.
Application 18/625,448 is a continuation of application No. PCT/US2022/045511, filed on Oct. 3, 2022.
Claims priority of provisional application 63/251,919, filed on Oct. 4, 2021.
Prior Publication US 2024/0285733 A1, Aug. 29, 2024
Int. Cl. A61K 38/44 (2006.01); A61P 31/04 (2006.01); A61P 37/04 (2006.01); C12N 9/02 (2006.01)
CPC A61K 38/44 (2013.01) [A61P 31/04 (2018.01); A61P 37/04 (2018.01); C12N 9/0008 (2013.01); C12Y 102/01003 (2013.01)] 18 Claims
 
1. A method of enhancing an immune response in an individual in need thereof, comprising administering:
[1] bacterial aldehyde dehydrogenase having at least 90% sequence identity to SEQ ID NO: 1 or SEQ ID NO: 2, a bacteria that produces said bacterial aldehyde dehydrogenase, or combinations thereof; and
[2] one or both of vitamin A and retinol at a concentration of from about 0.5 to 10 micromolar, or from about 0.75 to about 7.7 micromolar, or from about 1 to about 5 micromolar,
wherein said bacteria is selected from Candidatus Arthromitus, Bacillus bifidum, Bacillus cereus, Enterococcus faecalis, Bacillus subtilis, Clostridium perfringes, Escherichia Coli K-12, Staphylococcus warni, Lactobacillus acidophilus, Tissierellia bacterium, Bacteroidales bacterium, Caloranaerobacter azorensis, Keratinibaculum paraultunense, Aneurinibacillus sp., Bacteroidetes bacterium, Tissierella sp., Thermohalobacter berrensis, Clostridium sp., Syntrophomonadaceae bacterium, Clostridiales bacterium, Bacteroidales bacterium, Tenericutes bacterium, Paenibacillus assamensis, Bacillus kexueae, Saliterribacillus persicus, Margalitia camelliae, Senegalia massiliensis, Aquisalibacillus elongatus, Sporanaerobacter, Syntrophomonadaceae bacterium, Aquibacillus sediminis, Paludibacteraceae bacterium, Cytobacillus oceanisediminis, Robertmurraya spiralis, Peribacillus saganii, Gottschalkia purinilytica, Anaerostipes faecalis, Lederbergia citrisecundus, Cytobacillus firmus, Paenibacillus alvei, Margalitia shackletonii, Sporotomaculum syntrophicum, Paenibacillus arenosi, Tenericutes bacterium zrk29, Neobacillus mesonae, Romboutsia sp., Flavobacteriaceae bacterium Ap0902, Romboutsia, Neobacillus mesonae, Cytobacillus, oceanisediminis, Lederbergia citrisecundus, Neobacillus bataviensis, Halolactibacillus, alkaliphilus, Alkalihalobacillus wakoensis, Neobacillus massiliamazoniensis, Piscibacillus halophilus, Romboutsia, Bacillus dafuensis, Piscibacillus halophilus, Paenibacillus alvei, Gallicola sp., Saliterribacillus persicus, Arthrobacter citreus, Neobacillus vireti, Cytophagales bacterium, Anaerosalibacter massiliensis, Mollicutes bacterium, Neobacillus novalis, Methanosarcinaceae archaeon, and combinations thereof.