	US 12,138,291 B2
	Hemostatic composition and hemostatic method using hemostatic composition
Keiji Nagano, Tokyo (JP); and Satoru Kobayashi, Tokyo (JP)
Assigned to 3-D Matrix, Ltd., Tokyo (JP)
Filed by 3-D MATRIX, LTD., Tokyo (JP)
Filed on Mar. 17, 2022, as Appl. No. 17/697,459.
Application 17/697,459 is a continuation of application No. 16/060,269, granted, now 11,298,397, previously published as PCT/JP2016/087298, filed on Dec. 14, 2016.
Claims priority of application No. 2015-247334 (JP), filed on Dec. 18, 2015.
Prior Publication US 2022/0265757 A1, Aug. 25, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/04 (2006.01); A61K 9/12 (2006.01); A61K 9/70 (2006.01); A61K 38/00 (2006.01); A61K 38/08 (2019.01); A61K 38/10 (2006.01); A61L 26/00 (2006.01); A61P 7/04 (2006.01); A61P 17/02 (2006.01); C07K 7/00 (2006.01)

CPC A61K 38/04 (2013.01) [A61K 9/12 (2013.01); A61K 9/7015 (2013.01); A61K 38/00 (2013.01); A61K 38/08 (2013.01); A61K 38/10 (2013.01); A61L 26/0028 (2013.01); A61L 26/0076 (2013.01); A61P 7/04 (2018.01); A61P 17/02 (2018.01); C07K 7/00 (2013.01); A61L 2400/04 (2013.01)]

4 Claims

1. A spray apparatus loaded with a composition comprising the self-assembling peptide IEIK13 (SEQ ID NO:2) in solution,

wherein the concentration of IEIK13 (SEQ ID NO:2) in solution is 1.5% to 3% by weight;

wherein a pressure applied to the peptide solution by the spray apparatus is in the range between 0.5 kgf/cm²to 1.5 kgf/cm²; and

wherein the sprayed self-assembling peptide self-assembles after being sprayed onto a bleeding site of a subject, thereby forming a gel and preventing further bleeding from the site.