	US 12,137,650 B2
	Methods for promoting production of viable seeds from apomictic guayule plants
Eric Jan Mathur, Encinitas, CA (US); and Brett Tyler Roberts, San Diego, CA (US)
Assigned to AMERICAN BIORUBBER, LLC, Solana Beach, CA (US)
Filed by AMERICAN BIORUBBER, LLC, Solana Beach, CA (US)
Filed on Apr. 14, 2023, as Appl. No. 18/300,722.
Application 18/300,722 is a division of application No. 16/721,385, filed on Dec. 19, 2019, granted, now 11,672,216.
Application 18/300,722 is a division of application No. 15/571,001, abandoned, previously published as PCT/US2016/029850, filed on Apr. 28, 2016.
Claims priority of provisional application 62/156,148, filed on May 1, 2015.
Prior Publication US 2023/0247952 A1, Aug. 10, 2023
Int. Cl. A01H 1/02 (2006.01); A01H 5/02 (2018.01); A01H 6/14 (2018.01); C08J 5/02 (2006.01)

CPC A01H 1/022 (2021.01) [A01H 1/02 (2013.01); A01H 5/02 (2013.01); A01H 6/14 (2018.05); C08J 5/02 (2013.01); C08J 2321/02 (2013.01)]

4 Claims

1. A method for producing viable interploidy hybrid guayule seeds, said method comprising:

selecting an apomictic female guayule plant line and a pollinator plant line, wherein said apomictic female guayule plane line has a different ploidy level than said pollinator plant line;

determining a production ratio of female guayule plants of said apomictic female guayule plane line to pollinator plants of said pollinator plant line to produce said viable interploidy hybrid seeds, wherein said production ratio of female guayule plants to pollinator plants is in a range between 10:1 to 1:5;

planting said apomictic female guayule plant line and said pollinator plant line in pollinating proximity in said production ratio in a production field; and

allowing pollination of said female guayule plants with pollen from said pollinator plants, wherein at least 5% of said viable interploidy hybrid guayule seeds produced have germinated after incubation.