US 12,469,313 B2
Label free cell sorting
Jayant Parthasarathy, Excelsior, MN (US)
Assigned to Astrin Biosciences, Inc., St. Paul, MN (US)
Filed by ASTRIN BIOSCIENCES, INC., Excelsior, MN (US)
Filed on Aug. 3, 2022, as Appl. No. 17/879,929.
Claims priority of provisional application 63/229,175, filed on Aug. 4, 2021.
Prior Publication US 2023/0040252 A1, Feb. 9, 2023
Int. Cl. G06V 20/69 (2022.01); G01N 15/14 (2006.01); G01N 15/1433 (2024.01); G06T 7/00 (2017.01); G06V 10/774 (2022.01); G01N 15/10 (2006.01); G01N 15/149 (2024.01)
CPC G06V 20/698 (2022.01) [G01N 15/1433 (2024.01); G01N 15/1459 (2013.01); G06T 7/0012 (2013.01); G06V 10/774 (2022.01); G06V 20/695 (2022.01); G01N 2015/1006 (2013.01); G01N 15/149 (2024.01); G06T 2207/10056 (2013.01); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30096 (2013.01); G06T 2207/30204 (2013.01); G06T 2207/30242 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for sorting cells comprising:
receiving image data of a sample including multiple cells, the image data including multiple image frames, wherein each of the multiple image frames includes image data of a portion of the multiple cells included in the sample;
determining a flow rate for the sample;
generating a trigger for each of the multiple image frames based on timing data corresponding to a capture time for the image data included in each of the multiple image frames;
detecting a frame of interest within the multiple image frames based on an output of a classification model, the frame of interest including a cell of interest;
determining two adjacent image frames for the frame of interest based on the flow rate and the timing data, the two adjacent image frames including an image frame captured right before the frame of interest and an image frame captured right after the frame of interest; and
separating the cell of interest from the multiple cells included in the sample by actuating a mechanical device based on the trigger for the frame of interest and the trigger for each of the two adjacent image frames.