US 12,468,136 B2
Random access projection microscopy
Kaspar Podgorski, Ashburn, VA (US); and Daniel Flickinger, Frederick, MD (US)
Assigned to Howard Hughes Medical Institute, Ashburn, VA (US)
Appl. No. 17/755,836
Filed by Howard Hughes Medical Institute, Ashburn, VA (US)
PCT Filed Nov. 11, 2020, PCT No. PCT/US2020/070770
§ 371(c)(1), (2) Date May 10, 2022,
PCT Pub. No. WO2021/097482, PCT Pub. Date May 20, 2021.
Claims priority of provisional application 62/933,804, filed on Nov. 11, 2019.
Prior Publication US 2022/0382031 A1, Dec. 1, 2022
Int. Cl. G02B 21/00 (2006.01); G02B 21/06 (2006.01); G02B 26/08 (2006.01); G02B 26/12 (2006.01)
CPC G02B 21/0076 (2013.01) [G02B 21/06 (2013.01); G02B 26/0833 (2013.01); G02B 26/123 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A method of imaging a sample, the method comprising:
providing light from a light source;
directing the provided light into an extended focus of light;
scanning the extended focus of light across a wavefront modulating element a plurality of times, wherein the wavefront modulating element includes a pattern that modulates amplitudes of segments of the extended focus of light as the light is scanned;
providing different patterns on the wavefront modulating element during different times of the plurality of times that the extended focus is scanned across the wavefront modulating element, wherein the different patterns on the wavefront modulating element modulate amplitudes of segments of the extended focus of the light differently as the light is scanned;
providing the modulated light from the plurality of times the extended focus is scanned to the sample;
detecting light emitted from the sample in response to excitation by the modulated light; and
generating an image of the sample based on the detected light.