US 12,467,933 B2
Body fluid iron level panel analyzer
Erica Forzani, Mesa, AZ (US); David Jackemeyer, Tempe, AZ (US); and Michael Serhan, Tempe, AZ (US)
Assigned to Arizona Board of Regents on Behalf of Arizona State University, Tempe, AZ (US)
Appl. No. 17/616,562
Filed by Erica Forzani, Mesa, AZ (US); David Jackemeyer, Tempe, AZ (US); and Michael Serhan, Tempe, AZ (US)
PCT Filed Jun. 8, 2020, PCT No. PCT/US2020/036660
§ 371(c)(1), (2) Date Dec. 3, 2021,
PCT Pub. No. WO2020/247935, PCT Pub. Date Dec. 10, 2020.
Claims priority of provisional application 62/858,421, filed on Jun. 7, 2019.
Prior Publication US 2022/0236293 A1, Jul. 28, 2022
Int. Cl. G01N 33/90 (2006.01); B01L 3/00 (2006.01); G01N 21/77 (2006.01); G01N 21/78 (2006.01); G01N 21/84 (2006.01); G01N 33/52 (2006.01); G01N 33/80 (2006.01); G01N 33/84 (2006.01)
CPC G01N 33/90 (2013.01) [B01L 3/5023 (2013.01); B01L 3/502715 (2013.01); G01N 21/78 (2013.01); G01N 21/8483 (2013.01); G01N 33/525 (2013.01); G01N 33/80 (2013.01); G01N 33/84 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/069 (2013.01); G01N 2021/7759 (2013.01); G01N 2201/062 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A system for measuring the concentration of iron and total iron binding capacity in a body fluid sample, the system comprising:
a sensor strip comprising:
a first layer comprising a screening film operable to receive the body fluid sample;
a second layer adjacent to the first layer, the second layer comprising a first reagent for reducing iron (III) to iron (II) in the body fluid sample;
a third layer adjacent to the second layer, the third layer comprising a sensing area comprising a second reagent for chelating iron (II) to form a chromogen complex and a reference area without a reagent modification, wherein formation of the chromogen complex causes a color change to the sensor strip that correlates with the concentration of iron in the body fluid sample;
a fourth layer operable as a detection sink;
a fifth layer saturated with iron; and
a sixth layer saturated with magnesium carbonate for measuring total iron binding capacity; and
a processor configured for use in conjunction with the sensor strip, including instructions which, when executed, cause the processor to:
receive light intensity data comprising light intensities from the sensor strip after the body fluid sample is placed on the sensor strip and causes a color change to the sensor strip;
extract red-green-blue (RGB) component values or red spectrum light intensities from the light intensity data of the sensor strip;
calculate the absorbance of the RGB component values or red spectrum light intensities for the sensor strip; and
calculate iron concentration in the body fluid sample in the sensor strip from the absorbance of the RGB component values or red spectrum light intensity for the sensor strip.