US 12,467,922 B2
Hybridoma cell line secreting an ActA monoclonal antibody and use thereof
Xinan Jiao, Yangzhou (CN); Yuelan Yin, Yangzhou (CN); Mengdi Zhao, Yangzhou (CN); Fanzeng Meng, Yangzhou (CN); Xiang Chen, Yangzhou (CN); Jinlin Huang, Yangzhou (CN); Zhiming Pan, Yangzhou (CN); and Dan Gu, Yangzhou (CN)
Assigned to Yangzhou University, Yangzhou (CN)
Appl. No. 18/851,087
Filed by Yangzhou University, Yangzhou (CN)
PCT Filed May 30, 2023, PCT No. PCT/CN2023/097071
§ 371(c)(1), (2) Date Sep. 26, 2024,
PCT Pub. No. WO2023/186189, PCT Pub. Date Oct. 5, 2023.
Claims priority of application No. 202210339643.3 (CN), filed on Apr. 1, 2022.
Prior Publication US 2025/0110125 A1, Apr. 3, 2025
Int. Cl. G01N 33/569 (2006.01); C07K 16/12 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/56911 (2013.01) [C07K 16/1296 (2013.01); G01N 33/581 (2013.01); C07K 2317/94 (2013.01); G01N 2333/195 (2013.01); G01N 2333/908 (2013.01); G01N 2469/10 (2013.01); G01N 2470/10 (2021.08)] 5 Claims
OG exemplary drawing
 
1. A competitive ELISA detection kit for detecting Listeria monocytogenes infection, comprising an Actin assembly-inducing protein (ActA) monoclonal antibody or a polynucleotide;
wherein the ActA monoclonal antibody comprises a heavy chain and a light chain, wherein the light chain comprises three complementarity determining regions with amino acid sequences shown as SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, respectively; and the heavy chain comprises three complementarity determining regions with amino acid sequences shown as SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively;
wherein the polynucleotide encodes the ActA monoclonal antibody, wherein a nucleotide sequence encoding the light chain of the ActA monoclonal antibody is shown as SEQ ID NO: 8, and a nucleotide sequence encoding the heavy chain of the ActA monoclonal antibody is shown as SEQ ID NO: 10;
wherein the competitive ELISA detection kit further comprises one or more of the following: an enzyme-labeled antibody, a coated antigen, a positive and/or negative control serum for Listeria monocytogenes, a coating buffer, a blocking buffer, a dilution buffer, a washing buffer, a substrate solution, and a reaction stop solution.