US 12,467,920 B2
Nanocomposites and nanoagents for detection and treatment of a target of interest and methods of making and using same
Alexandru S. Biris, Little Rock, AR (US); and Zeid Nima, Little Rock, AR (US)
Assigned to BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US)
Filed by BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US)
Filed on Apr. 22, 2021, as Appl. No. 17/237,184.
Application 17/237,184 is a division of application No. 15/487,683, filed on Apr. 14, 2017, granted, now 11,016,089.
Application 15/487,683 is a continuation in part of application No. 14/921,927, filed on Oct. 23, 2015, granted, now 9,700,573, issued on Jul. 11, 2017.
Application 15/487,683 is a continuation in part of application No. 14/683,929, filed on Apr. 10, 2015, granted, now 9,810,687, issued on Nov. 7, 2017.
Application 15/487,683 is a continuation in part of application No. 14/683,978, filed on Apr. 10, 2015, granted, now 9,675,078, issued on Jun. 13, 2017.
Application 15/487,683 is a continuation in part of application No. 14/513,744, filed on Oct. 14, 2014, granted, now 9,784,737, issued on Oct. 10, 2017.
Application 15/487,683 is a continuation in part of application No. 14/181,981, filed on Feb. 17, 2014, abandoned.
Claims priority of provisional application 62/322,489, filed on Apr. 14, 2016.
Prior Publication US 2021/0311045 A1, Oct. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/553 (2006.01); A61K 9/00 (2006.01); A61K 31/337 (2006.01); A61K 31/704 (2006.01); A61K 41/00 (2020.01); A61K 47/68 (2017.01); A61K 47/69 (2017.01); A61K 49/00 (2006.01); B01J 13/02 (2006.01); B01J 13/06 (2006.01); B01J 13/20 (2006.01); B01J 13/22 (2006.01); B32B 15/02 (2006.01); B82Y 5/00 (2011.01); B82Y 15/00 (2011.01); B82Y 30/00 (2011.01); B82Y 40/00 (2011.01); G01N 33/531 (2006.01); G01N 33/574 (2006.01); H01F 1/00 (2006.01); A61N 1/40 (2006.01)
CPC G01N 33/553 (2013.01) [A61K 31/337 (2013.01); A61K 31/704 (2013.01); A61K 41/0052 (2013.01); A61K 47/6849 (2017.08); A61K 47/6923 (2017.08); A61K 49/0065 (2013.01); B01J 13/02 (2013.01); B01J 13/06 (2013.01); B01J 13/20 (2013.01); B01J 13/22 (2013.01); B32B 15/02 (2013.01); G01N 33/531 (2013.01); G01N 33/57492 (2013.01); H01F 1/0054 (2013.01); A61K 9/0092 (2013.01); A61N 1/406 (2013.01); B82Y 5/00 (2013.01); B82Y 15/00 (2013.01); B82Y 30/00 (2013.01); B82Y 40/00 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A method of making a nanocomposite for detection and treatment of a target of interest, wherein the target of interest comprises tumor cells or pathogens, comprising:
forming at least one nanostructure, each nanostructure having a core and a shell surrounding the core;
assembling a reporter on the shell of each nanostructure, wherein the reporter is adapted for respectively transmitting at least one signature from the target of interest; and
conjugating a layer of a treating agent and/or a targeting agent to the reporter, wherein the treating agent is adapted for treating the target of interest, and the targeting agent is adapted for targeting the nanocomposite to the target of interest,
wherein the step of assembling the reporter comprises:
dispersing the at least one nanostructure in distilled water to form a first mixture;
dissolving the reporter in ethanol to form a reporter solution;
adding the reporter solution to the first mixture and stirring to form a second mixture; and
centrifuging the second mixture to form a first precipitate comprising the at least one nanostructure assembled with the reporter; and
wherein the reporter comprises 4-mercaptobenzoic acid (4MBA), p-aminothiophenol (PATP), p-nitrothiophenol (PNTP), 4-(methylsulfanyl) thiophenol (4MSTP), molecules with a unique Raman spectral signature, or a fluorescent agent.