US 12,467,863 B2
Detection method and detection system using local surface plasmon resonance (LSPR) spectral image brightness contrast
Pei-Kuen Wei, Taipei (TW); Sheng-Hann Wang, Chaozhou Township, Pingtung County (TW); and Ting-Wei Chang, New Taipei (TW)
Assigned to ACADEMIA SINICA, Taipei (TW)
Filed by ACADEMIA SINICA, Taipei (TW)
Filed on Dec. 20, 2022, as Appl. No. 18/084,997.
Claims priority of provisional application 63/266,037, filed on Dec. 27, 2021.
Prior Publication US 2023/0204508 A1, Jun. 29, 2023
Int. Cl. G01N 21/552 (2014.01); G01N 21/17 (2006.01)
CPC G01N 21/554 (2013.01) [G01N 2021/177 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A detection method for detecting objects of interest attached to a surface of a plurality of reporters, comprising:
illuminating, by a light source, the plurality of reporters flowing in a microfluidic chip;
obtaining, by an image sensor, a plurality of local surface plasmon resonance (LSPR) spectral images of each of the plurality of the reporters individually, wherein each of the LSPR spectral images has a brightness of a long wavelength band (BA) and a brightness of a short wavelength band (BB);
calculating, by a processor, a spectral image brightness contrast y for each of the reporters to amplify a spectral shift caused by the reporters and thereby enhance detection sensitivity, wherein γ=(BA−BB)/(BA+BB);
calculating, by a processor, an absolute value |γ| of each of the spectral image brightness contrast γ; and
determining, by a processor, whether each of absolute value |γ| satisfies a defined positive threshold for |γ|>0.1.