US 12,467,837 B2
Solid phase extraction methods for enhanced removal of phospholipids from biological samples
Bonnie A. Alden, Whitinsville, MA (US); Kenneth Berthelette, Millville, MA (US); Jon Finch, Plymouth, MN (US); Donna Osterman, Northbridge, MA (US); Thomas Swann, Hopedale, MA (US); and Thomas H. Walter, Ashland, MA (US)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Feb. 9, 2023, as Appl. No. 18/166,738.
Application 18/166,738 is a continuation of application No. 16/230,567, filed on Dec. 21, 2018, granted, now 11,604,123.
Claims priority of provisional application 62/609,262, filed on Dec. 21, 2017.
Prior Publication US 2023/0204478 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 15/36 (2006.01); B01D 15/32 (2006.01); B01D 15/38 (2006.01); B01D 15/42 (2006.01); B01J 39/05 (2017.01); B01J 39/26 (2006.01); G01N 1/40 (2006.01); G01N 33/92 (2006.01); G01N 30/00 (2006.01); G01N 30/06 (2006.01)
CPC G01N 1/405 (2013.01) [B01D 15/327 (2013.01); B01D 15/362 (2013.01); B01D 15/3847 (2013.01); B01D 15/426 (2013.01); B01J 39/05 (2017.01); B01J 39/26 (2013.01); G01N 33/92 (2013.01); G01N 2030/009 (2013.01); G01N 2030/062 (2013.01); G01N 2405/00 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of performing a sample enrichment procedure, the method consisting of:
(a) adding a sample fluid that comprises at least one phospholipid and at least one target analyte to an ammonium-modified sorbent comprising a hydrophobic component and a cation exchange component, wherein the cation exchange component comprises sulfonate groups and the cation of the cation exchange component is ammonium (NH4+), thereby resulting in the ammonium-modified sorbent adsorbed with phospholipid and adsorbed with target analyte;
(b) adding an organic solution consisting of a first organic solvent species to the ammonium-modified sorbent thereby desorbing at least a portion of the phospholipid from the ammonium-modified sorbent; and
(c) adding an elution solution consisting of a basic compound or salt species and a second organic solvent species to the ammonium-modified sorbent, thereby desorbing at least a portion of the target analyte from the ammonium-modified sorbent and forming a solution of the target analyte in the elution solution.