US 12,467,101 B2
Compositions for detecting Candida species
Angela S. Hudson, San Diego, CA (US); Damon K. Getman, Poway, CA (US); Alice Jiang, San Diego, CA (US); and Barbara L. Eaton, San Diego, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Filed by Gen-Probe Incorporated, San Diego, CA (US)
Filed on Jul. 9, 2021, as Appl. No. 17/371,274.
Application 17/371,274 is a continuation of application No. 16/065,461, granted, now 11,111,549, previously published as PCT/US2017/012163, filed on Jan. 4, 2017.
Claims priority of provisional application 62/274,610, filed on Jan. 4, 2016.
Prior Publication US 2021/0340634 A1, Nov. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6895 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01); C12Q 1/689 (2018.01)
CPC C12Q 1/6895 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 1/689 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A reaction mixture for determining the presence or absence of Candida species (sp.) in a sample, wherein the Candida sp. is one or more of C. albicans, C. parapsilosis, C. dubliniensis, C. tropicalis, and C. glabrata, the reaction mixture comprising:
a first amplification oligomer combination and a second amplification oligomer combination, wherein
(a) the first amplification oligomer combination comprises first and second Candida-specific amplification oligomers for amplifying a first Candida sp. nucleic acid target region, wherein said first target region is a region of SEQ ID NO:129 from about nucleotide position 133 to about nucleotide position 259, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences, wherein
the first Candida-specific target-hybridizing sequence consists of the nucleotide sequence of residues 28-46 of SEQ ID NO:9; and
the second Candida-specific target-hybridizing sequence consists of the nucleotide sequence of SEQ ID NO:26; and
(b) the second amplification oligomer combination comprises first and second C. glabrata-specific amplification oligomers for amplifying a second Candida sp. nucleic acid target region, wherein said second target region is a region of SEQ ID NO:131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C. glabrata-specific amplification oligomers respectively comprise first and second C. glabrata-specific target-hybridizing sequences, wherein
the first C. glabrata-specific target-hybridizing sequence is a sequence of 15 to 24 contiguous nucleotides contained in the sequence of SEQ ID NO:134 and that includes at least the sequence of SEQ ID NO: 135; and/or
the second C. glabrata-specific target-hybridizing sequence is a sequence of 16 to 21 contiguous nucleotides contained in the sequence of SEQ ID NO:136 and that includes at least the sequence of SEQ ID NO:137;
wherein each of the first Candida-specific amplification oligomer and the first C. glabrata-specific amplification oligomer is a promoter primer or promoter provider further comprising a promoter sequence located 5′ to the respective target-hybridizing sequence, and
wherein each of the second Candida-specific amplification oligomer and the second C. glabrata-specific amplification oligomer is a non-promoter primer;
a Candida-specific detection probe comprising a target-hybridizing sequence that is complementary to the first Candida sp. target region and a C. glabrata-specific detection probe comprising a target-hybridizing sequence that is complementary to the second Candida sp. target region, wherein each of the Candida-specific and C. glabrata-specific detection probes is a molecular torch and comprises a fluorescent label and a quencher; and
an RNA polymerase.