	US 12,467,072 B2
	Method of improving biomass high-solid enzymatic hydrolysis rate
Mian Li, Santa Clara, CA (US); Zhiqian Liu, Quzhou (CN); Xuhao Zeng, Quzhou (CN); Changhui Hu, Quzhou (CN); Jing Wang, Quzhou (CN); Xue Cai, Quzhou (CN); Xiaojian Zhang, Quzhou (CN); and Yuguo Zheng, Quzhou (CN)
Assigned to Zhejiang Huakang Pharmaceutical Co., Ltd., Quzhou (CN); and Zhejiang University of Technology, Hangzhou (CN)
Appl. No. 18/023,067
Filed by ZHEJIANG HUAKANG PHARMACEUTICAL CO., LTD., Quzhou (CN); and ZHEJIANG UNIVERSITY OF TECHNOLOGY, Hangzhou (CN)
PCT Filed Nov. 30, 2021, PCT No. PCT/CN2021/134584 § 371(c)(1), (2) Date Feb. 24, 2023, PCT Pub. No. WO2022/116978, PCT Pub. Date Jun. 9, 2022.
Claims priority of application No. 202011403739.9 (CN), filed on Dec. 4, 2020.
Prior Publication US 2023/0295677 A1, Sep. 21, 2023
Int. Cl. C12P 19/14 (2006.01); C12N 9/42 (2006.01)

CPC C12P 19/14 (2013.01) [C12N 9/2437 (2013.01); C12Y 302/01004 (2013.01); C12P 2201/00 (2013.01)]

5 Claims

1. A method of improving a biomass high-solid enzymatic hydrolysis rate, comprising the following steps:

at step 1, obtaining a biomass substrate with a water content of 50% to 70% by weight by performing pretreatment on a biomass raw material, and screening the biomass substrate through a mesh screen to obtain a coarse-particle biomass substrate above 10 meshes and a fine-particle biomass substrate from 10 to 50 meshes;

at step 2, adjusting a water content of the fine-particle biomass substrate to 60% to 65% by weight and a water content of the coarse-particle biomass substrate to 50% to 70% by weight, and mixing the fine-particle biomass substrate and coarse-particle biomass substrate having different water contents to obtain a mixed substrate having a water content of 50% to 65% by weight;

at step 3, placing the mixed substrate into an enzymatic hydrolysis tank, and then adding a citrate buffer solution with pH 4.6 to 5.5 and a cellulase for enzymatic hydrolysis.