	US 12,467,070 B2
	Semisynthetic process for the preparation of colchicine
Fabio Donzelli, Milan (IT); Pietro Allegrini, Milan (IT); Alessandro Andreani, Milan (IT); Andrea Gambini, Milan (IT); and Davide Berlanda, Milan (IT)
Assigned to Indena S.P.A., Milan (IT)
Appl. No. 18/001,741
Filed by Indena S.P.A., Milan (IT)
PCT Filed Jun. 14, 2021, PCT No. PCT/IB2021/055213 § 371(c)(1), (2) Date Dec. 14, 2022, PCT Pub. No. WO2021/255618, PCT Pub. Date Dec. 23, 2021.
Claims priority of application No. 102020000014362 (IT), filed on Jun. 16, 2020.
Prior Publication US 2023/0227873 A1, Jul. 20, 2023
Int. Cl. C12P 15/00 (2006.01); C07C 233/32 (2006.01); C12N 9/24 (2006.01)

CPC C12P 15/00 (2013.01) [C07C 233/32 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01021 (2013.01)]

12 Claims

1. A process for the preparation of colchicine 1 from colchicoside 2 comprising

converting enzymatically colchicoside 2 to 3-O-demethylcolchicine 3, wherein enzymatic conversion is obtained with a cellulase, wherein the cellulase is a mixture of at least two or more enzymes, including at least one of β-glucosidase (EC 3.2.1.21), endo-(1,4)-β-D-glucanase (EC 3.2.1.4) and exo-(1,4)-β-D-glucanase (EC 3.2.1.91).