US 12,467,069 B2
Cis-aconitate synthesis enzyme and uses thereof
Gyoo Yeol Jung, Pohang-si (KR); Jae Seong Yang, Pohang-si (KR); Myung Hyun Noh, Pohang-si (KR); and Dae Yeol Ye, Pohang-si (KR)
Assigned to POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION, Pohang-si (KR)
Filed by POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION, Pohang-si (KR)
Filed on Mar. 30, 2023, as Appl. No. 18/192,868.
Claims priority of application No. 10-2022-0042345 (KR), filed on Apr. 5, 2022; and application No. 10-2022-0189584 (KR), filed on Dec. 29, 2022.
Prior Publication US 2023/0313239 A1, Oct. 5, 2023
Int. Cl. C12P 7/46 (2006.01); C12N 1/20 (2006.01); C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01)
CPC C12P 7/46 (2013.01) [C12N 1/20 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12Y 401/01006 (2013.01); C12Y 402/01079 (2013.01); C12N 2800/101 (2013.01)] 14 Claims
 
1. An expression cassette for introducing an itaconate production pathway comprising a 2-methylcitrate dehydratase (PrpD) gene represented by a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1, and 7 to 12, and further comprising a nucleotide sequence of SEQ ID NO: 17.