US 12,467,068 B2
Process for the production and recovery of bio-surfactant from Bacillus sp. MCC0156
Syed Gulam Dastager, Pune (IN); and Hari Ram, Pune (IN)
Assigned to COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH, New Delhi (IN)
Appl. No. 17/429,336
Filed by COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH, New Delhi (IN)
PCT Filed Feb. 4, 2020, PCT No. PCT/IN2020/050105
§ 371(c)(1), (2) Date Aug. 7, 2021,
PCT Pub. No. WO2020/161735, PCT Pub. Date Aug. 13, 2020.
Claims priority of application No. 201911005440 (IN), filed on Feb. 8, 2019.
Prior Publication US 2022/0193739 A1, Jun. 23, 2022
Int. Cl. C12P 7/04 (2006.01); C12N 1/02 (2006.01); C12N 1/20 (2006.01); C12P 1/04 (2006.01); C12P 5/02 (2006.01); B09C 1/10 (2006.01)
CPC C12P 5/026 (2013.01) [C12N 1/02 (2013.01); C12N 1/20 (2013.01); B09C 1/10 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A process for production and recovery of a bio surfactant mixture consisting of 1-pentanonacontene and 3-hydroxy-16-methyl heptadecanoic acid from Bacillus sp. MCC0156 comprising the following steps:
a) inoculating inoculum from overnight grown culture of Bacillus sp. MCC0156 in a medium consisting of yeast extract in water;
b) obtaining a cell free supernatant by centrifuging;
c) regulating pH of the cell free supernatant of step (b) to 2.0 using an acid and storing overnight at 4° C. to obtain an acid precipitated pellet by centrifugation and dissolving in water, and adjusting to pH of 8.0;
d) repeating step of acid precipitation of step (c) at least two times to remove impurities from the medium to obtain a crude bio-surfactant mixture; and
e) freeze drying the acid precipitated pellet to obtain a lyophilized bio surfactant mixture consisting of 1-pentanonacontene and 3-hydroxy-16-methyl heptadecanoic.