	US 12,467,067 B2
	Cell transfection
Aliah Mohammed Abuammah, Riyadh (SA)
Appl. No. 17/432,405
Filed by Aliah Mohammed Abuammah, Riyadh (SA)
PCT Filed Feb. 20, 2020, PCT No. PCT/GB2020/050406 § 371(c)(1), (2) Date Aug. 19, 2021, PCT Pub. No. WO2020/169979, PCT Pub. Date Aug. 27, 2020.
Claims priority of application No. 119400475 (SA), filed on Feb. 20, 2019; application No. 119400476 (SA), filed on Feb. 20, 2019; and application No. 119400477 (SA), filed on Feb. 20, 2019.
Prior Publication US 2022/0186262 A1, Jun. 16, 2022
Int. Cl. C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/90 (2006.01)

CPC C12N 15/907 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/113 (2013.01); C12N 2310/14 (2013.01); C12N 2310/20 (2017.05); C12N 2533/52 (2013.01); C12N 2800/80 (2013.01)]

11 Claims

1. A method of preparing a surface for deposition of cells, the method comprising the steps of:

a) providing a biological material composition having a temperature of from 15 to 25° C.;

b) providing the surface having a temperature of from 30 to 50° C.; and

c) depositing the biological material composition having a temperature of from 15 to 25° C. onto the surface having a temperature of from 26 to 50° C., to at least partially adhere the biological material composition to the surface,

wherein the difference in temperature of the biological material composition and the surface in step c) is at least 10° C.