	US 12,467,059 B2
	*Rice white leaf and panicle gene WLP3* and application thereof in rice stress resistance and yield increase**
Yuchun Rao, Jinhua (CN); Tao Lu, Jinhua (CN); Jiahui Huang, Jinhua (CN); Wenjing Yin, Jinhua (CN); Qianqian Zhong, Jinhua (CN); Yuqi Yang, Jinhua (CN); Tianqi Lu, Jinhua (CN); Jinglei Sun, Jinhua (CN); and Qiwei Jia, Jinhua (CN)
Assigned to Zhejiang Normal University, Jinhua (CN)
Filed by Zhejiang Normal University, Jinhua (CN)
Filed on Jun. 9, 2023, as Appl. No. 18/331,970.
Application 18/331,970 is a continuation of application No. PCT/CN2023/086900, filed on Apr. 7, 2023.
Claims priority of application No. 202211494431.9 (CN), filed on Nov. 25, 2022.
Prior Publication US 2024/0175043 A1, May 30, 2024
Int. Cl. C12N 15/00 (2006.01); C07K 14/415 (2006.01); C12N 15/82 (2006.01)

CPC C12N 15/8273 (2013.01) [C07K 14/415 (2013.01); C12N 15/8262 (2013.01); C12N 15/8269 (2013.01)]

1 Claim

1. A method of expressing cold tolerance genes of rice plants comprising the step of mutating a rice white leaf and panicle gene WLP3, wherein the mutation results in a single base substitution in SEQ ID NO: 3 where nucleotide T at position 380 is changed to C, and the encoded protein has the amino acid sequence of SEQ ID NO: 2, and treating the rice plants to a temperature of 4° C. for 7 days; wherein the rice plants are japonica rice variety Zhonghua 11 (ZH11); and wherein expression levels of genes DREB1A, DREB1B, and DREB2B are increased in the rice plants compared to expression levels of DREB1A, DREB1B, and DREB2B in wild type ZH11 rice plants treated to a temperature of 4° C. for 7 days.