	US 12,467,055 B2
	Delivery of biological molecules to plant cells
Kacey Leigh Angacian, Medford, MA (US); Michelle Ming Yu Heeney, Hopkinton, MA (US); John Paul Issa, Medford, MA (US); and Adam Patrick Joyce, Stow, MA (US)
Assigned to INARI AGRICULTURE TECHNOLOGY, INC., Cambridge, MA (US)
Appl. No. 17/758,250
Filed by INARI AGRICULTURE TECHNOLOGY, INC., Cambridge, MA (US)
PCT Filed Dec. 29, 2020, PCT No. PCT/US2020/067255 § 371(c)(1), (2) Date Jun. 30, 2022, PCT Pub. No. WO2021/138288, PCT Pub. Date Jul. 8, 2021.
Claims priority of provisional application 62/955,700, filed on Dec. 31, 2019.
Prior Publication US 2023/0022635 A1, Jan. 26, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)

CPC C12N 15/8206 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/8213 (2013.01); C12N 2310/20 (2017.05)]

21 Claims

1. A method of delivering a biological material to the interior of a plant cell, the method comprising:

pre-treating the plant cell by contacting the plant cell with an ionic liquid comprising tetrabutylammonium acetate (TBAA);

contacting the pre-treated plant cell with a complex comprising the biological material and a fluorous agent without TBAA, wherein the fluorous agent does not consist of a perfluorocarbon,

wherein the biological material is delivered to the interior of the plant cell, wherein the plant cell is a maize or wheat plant cell, and

wherein the complex comprising the biological material and the fluorous agent is provided in a liquid, in micelles, or in an emulsion.