	US 12,467,054 B2
	Translation enhancer, template nucleic acid, production method of translation template, and production method of protein
Hiroaki Tada, Tokushima (JP); and Masataka Minami, Hyogo (JP)
Assigned to NUProtein Co., Ltd., Tokushima (JP)
Appl. No. 17/269,186
Filed by NUProtein Co., Ltd., Tokushima (JP)
PCT Filed Sep. 24, 2020, PCT No. PCT/JP2020/035949 § 371(c)(1), (2) Date Feb. 17, 2021, PCT Pub. No. WO2021/070616, PCT Pub. Date Apr. 15, 2021.
Claims priority of application No. 2019-187039 (JP), filed on Oct. 10, 2019.
Prior Publication US 2022/0333121 A1, Oct. 20, 2022
Int. Cl. C12N 15/67 (2006.01); C12P 21/02 (2006.01)

CPC C12N 15/67 (2013.01) [C12P 21/02 (2013.01)]

18 Claims

1. A translation enhancer used in a cell-free protein synthesis system, the translation enhancer consisting of a nucleic acid as a 3′ untranslated region linked adjacent to a 3′ terminus of a code region that encodes an amino acid sequence of a target protein,

wherein the 3′ untranslated region consists of:

a first region consisting of a sequence of 10 to 40 nucleic acids adjacent to the 3′ terminus of the code region, and

a second region consisting of a poly-A sequence having continuous 2 to 20 “A”s directly linked to the first region, and

wherein the first region has a hairpin structure,

the 3′ terminus of the first region is not “A”, and

no additional nucleic acid is linked to the 3′ terminus of the second region.