	US 12,467,050 B2
	Methods and compositions for engineering synthetic bioswitches for remote control of biological activity
Gabriel A. Kwong, Atlanta, GA (US); Ian C. Miller, Atlanta, GA (US); and Marielena Gamboa, Atlanta, GA (US)
Assigned to Georgia Tech Research Corporation, Atlanta, GA (US)
Appl. No. 16/652,905
Filed by Georgia Tech Research Corporation, Atlanta, GA (US)
PCT Filed Oct. 2, 2018, PCT No. PCT/US2018/053962 § 371(c)(1), (2) Date Apr. 1, 2020, PCT Pub. No. WO2019/070704, PCT Pub. Date Apr. 11, 2019.
Claims priority of provisional application 62/566,670, filed on Oct. 2, 2017.
Prior Publication US 2020/0299686 A1, Sep. 24, 2020
Int. Cl. C12N 15/11 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61K 48/00 (2006.01); C07K 14/705 (2006.01); C12N 15/86 (2006.01); C07K 14/725 (2006.01); C12N 15/63 (2006.01)

CPC C12N 15/11 (2013.01) [A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); A61K 48/005 (2013.01); C07K 14/70596 (2013.01); C12N 15/86 (2013.01); A61K 48/0066 (2013.01); A61K 2239/23 (2023.05); A61K 2239/31 (2023.05); A61K 2239/48 (2023.05); C07K 14/7051 (2013.01); C12N 15/635 (2013.01); C12N 2320/50 (2013.01); C12N 2740/15043 (2013.01); C12N 2830/001 (2013.01); C12N 2830/002 (2013.01)]

40 Claims

1. A first nucleic acid molecule comprising:

a first synthetic bioswitch; and

a first heterologous nucleic acid;

wherein the first synthetic bioswitch is operably linked to the first heterologous nucleic acid and comprises a first control element that is regulated by a first stimulus; and

wherein the first synthetic bioswitch comprises the sequence of SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 49, or SEQ ID NO: 50.