US 12,467,048 B2
Methods for mapping personalized translatome
Qianru Li, Evanston, IL (US); Haiwang Yang, Evanston, IL (US); and Zhe Ji, Evanston, IL (US)
Assigned to Northwestern University, Evanston, IL (US)
Filed by Northwestern University, Evanston, IL (US)
Filed on Dec. 6, 2021, as Appl. No. 17/543,476.
Claims priority of provisional application 63/121,680, filed on Dec. 4, 2020.
Prior Publication US 2022/0213468 A1, Jul. 7, 2022
Int. Cl. C12Q 1/68 (2018.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/63 (2006.01)
CPC C12N 15/1065 (2013.01) [C12N 9/22 (2013.01); C12N 15/1096 (2013.01); C12Y 301/27008 (2013.01); C12N 2800/80 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method of RNAse footprinting comprising: (a) contacting a biological sample comprising cells with an RNAse in an amount sufficient to selectively protect first RNA fragments bound to a translational ribosome in a coding region, while digesting each of second RNA fragments bound by a RNA binding protein other than the translation ribosome in the coding region, (b) sequencing the first RNA fragments, and (c) mapping a ribosome profile for each of the first RNA fragments, wherein the number of cells in the biological sample is less than about 100,000, and wherein said method does not comprise ribosome isolation by centrifugation or immunoprecipitation.