	US 12,467,046 B2
	Redirection of tropism of AAV capsids
Mathieu E. Nonnenmacher, Boston, MA (US); Jinzhao Hou, Lexington, MA (US); and Wei Wang, Lexington, MA (US)
Assigned to VOYAGER THERAPEUTICS, INC., Lexington, MA (US)
Appl. No. 17/282,479
Filed by VOYAGER THERAPEUTICS, INC., Lexington, MA (US)
PCT Filed Oct. 2, 2019, PCT No. PCT/US2019/054345 § 371(c)(1), (2) Date Apr. 2, 2021, PCT Pub. No. WO2020/072683, PCT Pub. Date Apr. 9, 2020.
Claims priority of provisional application 62/839,883, filed on Apr. 29, 2019.
Claims priority of provisional application 62/740,310, filed on Oct. 2, 2018.
Prior Publication US 2021/0380969 A1, Dec. 9, 2021
Int. Cl. C12N 15/10 (2006.01); C07K 14/005 (2006.01)

CPC C12N 15/1037 (2013.01) [C07K 14/005 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14145 (2013.01)]

29 Claims

1. A method for generating an adeno-associated virus (AAV) vector library encoding variant AAV capsid polypeptides, the method comprising:

(a) providing first nucleic acids comprising a P40 promoter and a cell-type specific promoter, wherein the cell-type specific promoter drives capsid mRNA expression in the absence of helper virus co-infection; and second nucleic acids encoding the variant AAV capsid polypeptides having a region of randomized sequence of 2, 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids; and

(b) cloning the first nucleic acids and the second nucleic acids under conditions suitable to generate the AAV vector library.