	US 12,467,044 B2
	Mutant aminoacyl tRNA synthetase
Lei Wang, San Francisco, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Aug. 10, 2022, as Appl. No. 17/818,919.
Application 17/818,919 is a continuation of application No. 16/484,757, granted, now 11,447,764, previously published as PCT/US2018/018063, filed on Feb. 13, 2018.
Claims priority of provisional application 62/458,557, filed on Feb. 13, 2017.
Prior Publication US 2022/0411778 A1, Dec. 29, 2022
Int. Cl. C12N 9/88 (2006.01); C07F 9/12 (2006.01); C07F 9/24 (2006.01); C12N 9/00 (2006.01); C12N 15/52 (2006.01); C12N 15/63 (2006.01)

CPC C12N 9/88 (2013.01) [C07F 9/12 (2013.01); C07F 9/242 (2013.01); C12N 9/93 (2013.01); C12N 15/52 (2013.01); C12N 15/63 (2013.01); C12N 2310/314 (2013.01); C12Y 601/01026 (2013.01)]

5 Claims

1. A mutant aminoacyl tRNA synthetase, comprising

(a) a mutant Methanosarcina mazei pyrrolysyl-tRNA synthetase having a sequence with at least 95% identity to SEQ ID NO: 2 and comprising serine at amino acid position 302, methionine at amino acid position 309, leucine at amino acid position 322, alanine at amino acid position 346, glycine at amino acid position 348, and threonine at amino acid position 417; or

(b) a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase having a sequence with at least 95% identity to SEQ ID NO: 3 and comprising serine at amino acid position 267, methionine at amino acid position 274, leucine at amino acid position 287, alanine at amino acid position 311, glycine at amino acid position 313, and threonine at amino acid position 382.