	US 12,467,035 B2
	Method of differentiating mesenchymal stem cells into cardiomyocytes
Youngkeun Ahn, Gwangju (KR); Yong Sook Kim, Gwangju (KR); Hye Jin Kang, Gwangju (KR); and Dong Im Cho, Gwangju (KR)
Assigned to CHONNAM NATIONAL UNIVERSITY HOSPITAL, Gwangju (KR)
Filed by CHONNAM NATIONAL UNIVERSITY HOSPITAL, Gwangju (KR)
Filed on Dec. 26, 2017, as Appl. No. 15/854,017.
Claims priority of application No. 10-2016-0179624 (KR), filed on Dec. 27, 2016.
Prior Publication US 2018/0201903 A1, Jul. 19, 2018
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/077 (2010.01); A61K 35/34 (2015.01)

CPC C12N 5/0657 (2013.01) [A61K 35/34 (2013.01); C12N 2500/30 (2013.01); C12N 2500/34 (2013.01); C12N 2501/065 (2013.01); C12N 2501/999 (2013.01); C12N 2506/1353 (2013.01); C12N 2510/00 (2013.01)]

5 Claims

1. A method of inducing differentiation of mesenchymal stem cells into cardiomyocytes, the method comprising treating adult mesenchymal stem cells by culturing the adult mesenchymal stem cells in a medium consisting of glucose-free basal culture medium and apicidin or in a medium consisting of glucose-free basal culture medium, apicidin and lactic acid, wherein apicidin induces differentiation of mesenchymal stem cells into cardiomyocytes,

then washing the cultured cells to produce cardiomyocytes, wherein the culturing is performed for 4 to 27 hours and wherein the expression of cardiomyocyte-specific markers is increased in the cardiomyocytes compared to the adult mesenchymal stem cells.