	US 12,467,034 B2
	Method for generating cells of the T cell lineage
Juan Carlos Zuniga-Pflucker, Toronto (CA); Mahmood Mohtashami, Toronto (CA); and Ashton Trotman-Grant, Toronto (CA)
Assigned to Sunnybrook Research Institute, Toronto (CA)
Appl. No. 16/969,854
Filed by Sunnybrook Research Institute, Toronto (CA)
PCT Filed Feb. 14, 2019, PCT No. PCT/CA2019/050181 § 371(c)(1), (2) Date Aug. 13, 2020, PCT Pub. No. WO2019/157597, PCT Pub. Date Aug. 22, 2019.
Claims priority of provisional application 62/630,497, filed on Feb. 14, 2018.
Prior Publication US 2020/0399599 A1, Dec. 24, 2020
Int. Cl. A61K 40/11 (2025.01); A61K 40/40 (2025.01); A61K 48/00 (2006.01); C12N 5/0783 (2010.01)

CPC C12N 5/0636 (2013.01) [A61K 40/11 (2025.01); A61K 40/40 (2025.01); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); C12N 2501/42 (2013.01); C12N 2506/45 (2013.01)]

5 Claims

1. A method of generating a population of progenitor T (proT) cells comprising culturing in suspension hematopoietic stem progenitor cells (HSPCs) with a Notch ligand conjugated to a suspension support in a non-conditioned medium to generate proT cells, wherein the Notch ligand is delta-like ligand 4 (DL4), and the suspension support is a particle or a microbead, wherein the non-conditioned medium is free of stromal cells, and wherein culturing the HSPCs with the Notch ligand conjugated to the suspension support inhibits the generation of CD19+ B lineage cells and CD11b+ myeloid cells.