US 12,467,029 B2
Systems and methods for autologous biological therapeutics
David Karli, Edwards, CO (US); and Theodore Sand, Eagle, CO (US)
Assigned to David Karli, Avon, CO (US)
Filed by Greyledge Technologies, LLC, Edwards, CO (US)
Filed on Dec. 10, 2017, as Appl. No. 15/836,884.
Application 15/836,884 is a continuation in part of application No. 14/848,073, filed on Sep. 8, 2015, granted, now 9,840,690.
Application 14/848,073 is a continuation of application No. 13/421,728, filed on Mar. 15, 2012, granted, now 9,164,079.
Claims priority of provisional application 61/453,658, filed on Mar. 17, 2011.
Prior Publication US 2018/0100133 A1, Apr. 12, 2018
Int. Cl. C12M 1/26 (2006.01); A61K 35/16 (2015.01); A61K 35/19 (2015.01); A61K 35/28 (2015.01); A61M 1/38 (2006.01); C12M 1/34 (2006.01); G01N 33/49 (2006.01)
CPC C12M 33/10 (2013.01) [A61K 35/16 (2013.01); A61K 35/19 (2013.01); A61K 35/28 (2013.01); A61M 1/382 (2013.01); C12M 41/36 (2013.01); G01N 33/491 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method of preparing a platelet rich plasma preparation using a cell concentrating system:
the cell concentration system comprising:
a blood separation component having a blood sample input and having a centrifuge to separate the blood sample into a red blood cell fraction and a plasma fraction, the plasma fraction comprising a target cell-rich fraction and a target cell-poor fraction;
a first vessel for storing the target cell-poor fraction separated from the blood sample;
a second vessel for storing the target cell-rich fraction separated from the blood sample;
a first valve upstream of the first vessel and the second vessel, and downstream of the blood separation component;
a second valve for providing selective fluid communication between the first vessel and the second vessel; and
a concentration and flow logic and control component comprising a concentration measurement component, a partial separation component, and a processor, the concentration measurement component comprising at least one of a fluorescent activated cell sorting component and an optical sensor, the partial separation component comprising a microfluidic channel device for separating particles within the blood sample and a light scattering device for measuring a number of particles in the blood sample;
the method comprising:
flowing a platelet rich plasma sample from the blood separation component through a flow meter, a hematology analyzer module, and the first valve into the first vessel;
flowing fluid from the first vessel to the second vessel via actuation of the second valve, an amount of the fluid from the first vessel being determined by logic executed by the processor, the logic comprising:
quantifying an amount of platelets in the platelet rich plasma sample;
quantifying an amount of a second cellular component in the platelet rich plasma sample;
determining a ratio of the platelets to the second cellular component to determine a therapeutic potential of the platelet rich plasma preparation that comprises the platelet rich plasma sample; and
responsive to a determination by the processor that the ratio of platelets to the second cellular component is below a threshold value, determining the amount of fluid from the first vessel that will add an amount of platelets to the platelet rich plasma sample to increase the ratio of platelets of the second cellular component above the threshold value; and
actuating the second valve to add the amount of fluid from the first vessel to the second vessel to form the platelet rich plasma preparation.