US 12,467,028 B2
Millifluidic device for advanced cultures of biological agents
Carmen Giordano, Milan (IT); Manuela Teresa Raimondi, Milan (IT); Luca Izzo, Roe' Volciano (IT); Matteo Lagana, Longone al Segrino (IT); Diego Albani, Limbiate (IT); and Paola Petrini, Milan (IT)
Assigned to POLITECNICO DI MILANO, Milan (IT)
Appl. No. 17/760,811
Filed by POLITECNICO DI MILANO, Milan (IT)
PCT Filed Sep. 8, 2020, PCT No. PCT/IB2020/058339
§ 371(c)(1), (2) Date Mar. 16, 2022,
PCT Pub. No. WO2021/053458, PCT Pub. Date Mar. 25, 2021.
Claims priority of application No. 102019000016376 (IT), filed on Sep. 16, 2019.
Prior Publication US 2022/0340854 A1, Oct. 27, 2022
Int. Cl. C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/42 (2006.01); C12M 3/06 (2006.01)
CPC C12M 29/10 (2013.01) [C12M 23/16 (2013.01); C12M 23/58 (2013.01); C12M 29/04 (2013.01); C12M 35/02 (2013.01); C12M 41/46 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A millifluidic device for cultures of biological agents comprising:
a main body (11) comprising at least one chamber with an independent hydraulic circuit, the at least one chamber including a first hole (40) closed at a bottom;
a separator (35);
a membrane (36) fixed to said separator (35);
a plug (15) closing said first hole (40);
said separator (35) designed to be placed in said first hole (40);
said separator (35) being extractable from said first hole (40) once the plug (15) is removed;
said membrane (36) divides said first hole (40) into an upper half-chamber and a lower half-chamber;
a pair of tubes (25) to perfuse said lower half-chamber;
a pair of tubes (26) to perfuse said upper half-chamber;
a first slide (22) placed centrally on said plug (15) and adapted to allow a complete view of an inside of the at least one chamber;
a second slide (42) placed centrally on said first hole (40) and adapted to allow a complete view of the inside of the at least one chamber;
said first hole (40) is closed at the bottom by said second slide (42);
a cylindrical body (41) rises from said first hole (40) and said second slide (42) is placed on the top of said cylindrical body (41);
said cylindrical body (41) has a second hole (43) coaxial to said cylindrical body (41), and said second hole (43) is open at the bottom and closed at the top by the second slide (42), wherein:
said pairs of tubes (25) and (26) are laterally aligned with said second hole (43) and transversely to said main body (11);
the lower half-chamber is delimited at the top by the membrane (36), at the bottom by the slide (42), and at the sides by a lower component (21) of the plug (15) or by the separator (35); and
the upper half-chamber is delimited at the top by the first slide (22), at the bottom by the membrane (36) and at the sides by the lower component (21) of the plug (15) or by the separator (35).