| CPC C12M 25/14 (2013.01) [C12M 1/18 (2013.01); C12M 23/06 (2013.01); C12M 23/20 (2013.01); C12M 23/22 (2013.01); C12M 23/28 (2013.01); C12M 23/44 (2013.01); C12M 25/02 (2013.01); C12M 25/06 (2013.01); C12M 25/16 (2013.01); C12M 41/46 (2013.01); C12N 5/0068 (2013.01); C12N 5/06 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01)] | 19 Claims |

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1. An apparatus for culturing cells, comprising:
a bioreactor including a matrix assembly for growing cells in connection with a cell medium flowing through the matrix assembly, the matrix assembly comprising at least one woven mesh layer and a plurality of non-woven layers, each of the non-woven layers being adapted for cell immobilization and being fluid permeable;
wherein the plurality of non-woven layers comprise at least a first non-woven layer in direct contact, over an entirety of said first non-woven layer, with at least a second non-woven layer.
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