US 12,467,026 B2
Cell culture tool and method for producing cell culture tool
Junichi Matsumoto, Kyoto (JP)
Assigned to Kataoka Corporation, Kyoto (JP)
Appl. No. 17/282,065
Filed by KATAOKA CORPORATION, Kyoto (JP)
PCT Filed Sep. 30, 2019, PCT No. PCT/JP2019/038605
§ 371(c)(1), (2) Date Apr. 1, 2021,
PCT Pub. No. WO2020/071332, PCT Pub. Date Apr. 9, 2020.
Claims priority of application No. 2018-186940 (JP), filed on Oct. 1, 2018; and application No. 2019-176857 (JP), filed on Sep. 27, 2019.
Prior Publication US 2021/0340482 A1, Nov. 4, 2021
Int. Cl. C12M 1/12 (2006.01); C12M 1/00 (2006.01)
CPC C12M 25/12 (2013.01) [C12M 25/18 (2013.01); C12M 31/00 (2013.01)] 14 Claims
 
1. A cell culture tool including:
a vessel including a flat bottom surface,
a cell culture base layer that contains a cell culture base, and
a photo-thermal conversion layer configured to convert light into heat,
wherein
the photo-thermal conversion layer is disposed on the flat bottom surface,
the cell culture base layer is disposed on the photo-thermal conversion layer,
a cell is not disposed on the cell culture base layer,
the cell culture base layer has a cell adhesive area to which a cell is adherable and a cell adhesion inhibitory area where cell adhesion is inhibited,
the cell adhesion inhibitory area includes a heat-denatured product of the cell culture base,
the cell culture base is an extracellular matrix, and
the flat bottom surface comprises a single flat bottom surface, an entirety of the vessel having the single flat bottom surface.