	US 12,466,862 B2
	Plant-produced mABs against chikungunya virus with enhanced effector function and efficacy
Qiang Chen, Chandler, AZ (US); Jonathan Hurtado, Chandler, AZ (US); Haiyan Sun, Chandler, AZ (US); and Huafang Lai, Chandler, AZ (US)
Assigned to Arizona Board of Regents on Behalf of Arizona State University, Tempe, AZ (US)
Appl. No. 17/615,803
Filed by Qiang Chen, Chandler, AZ (US); Jonathan Hurtado, Chandler, AZ (US); Haiyan Sun, Chandler, AZ (US); and Huafang Lai, Chandler, AZ (US)
PCT Filed Jun. 4, 2020, PCT No. PCT/US2020/036136 § 371(c)(1), (2) Date Dec. 1, 2021, PCT Pub. No. WO2020/247642, PCT Pub. Date Dec. 10, 2020.
Claims priority of provisional application 62/857,081, filed on Jun. 4, 2019.
Prior Publication US 2022/0298211 A1, Sep. 22, 2022
Int. Cl. C07K 14/415 (2006.01); C07K 16/10 (2006.01); C12N 15/82 (2006.01)

CPC C07K 14/415 (2013.01) [C07K 16/1081 (2013.01); C12N 15/8258 (2013.01); C07K 2317/13 (2013.01); C07K 2317/41 (2013.01); C07K 2317/732 (2013.01); C07K 2317/76 (2013.01)]

11 Claims

1. A glycosylated IgG monoclonal antibody having specificity to Chikungunya virus (CHIKV) E1 or E2 protein, comprising a heavy chain and a light chain pair selected from:

(a) a heavy chain comprising the amino acid sequence of SEQ. ID NO: 3; and a light chain comprising the amino acid sequence of SEQ. ID NO: 5;

(b) a heavy chain comprising the amino acid sequence of SEQ. ID NO: 7; and a light chain comprising the amino acid sequence of SEQ. ID NO: 9;

(c) a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 11; and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 13; or

(d) a heavy chain variable domain comprising the amino acid sequence of SEQ ID NO: 15; and a light chain variable domain comprising the amino acid sequence of SEQ ID NO: 17;

wherein the Fc domain of the heavy chain is characterized by an N-linked glycan profile comprising an N-linked GlcNAc2Man3GlcNAc2 (GnGn) core glycan (Man=Mannose, GlcNAc=N-acetylglucosamine) with more than about 95% uniformity.