	US 12,466,858 B2
	Helper plasmid and method for preparing recombinant adeno-associated virus
Huapeng Li, Guangdong (CN); Jia Liu, Guangdong (CN); and Wanwen Xie, Guangdong (CN)
Assigned to PACKGENE BIOTECH CO., LTD., Guangzhou (CN)
Filed by PACKGENE BIOTECH CO., LTD., Guangdong (CN)
Filed on Oct. 8, 2023, as Appl. No. 18/377,827.
Application 18/377,827 is a continuation of application No. PCT/CN2022/078621, filed on Mar. 1, 2022.
Claims priority of application No. 202110377907.X (CN), filed on Apr. 8, 2021.
Prior Publication US 2024/0076319 A1, Mar. 7, 2024
Int. Cl. C07K 14/005 (2006.01); C12N 7/00 (2006.01); C12N 15/63 (2006.01)

CPC C07K 14/005 (2013.01) [C12N 7/00 (2013.01); C12N 15/63 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01)]

8 Claims

1. A helper plasmid for preparing a recombinant adeno-associated virus (rAAV), comprising:

(1) a coding sequence of an AAV Rep protein and a coding sequence of an AAV Cap protein;

(2) at least one promoter sequence; and

(3) at least one DA′ sequence or AD′ sequence, wherein the AD′ sequence is a reverse complementary sequence of the DA′ sequence;

wherein the DA′ sequence is set forth in SEQ ID NO: 4; the AD′ sequence is set forth in SEQ ID NO: 3,

wherein the at least one promoter sequence is a sequence of a P5 promoter, and

the sequence of the P5 promoter is set forth in SEQ ID NO: 2.