US 12,465,917 B2
Nucleic acid integrated detection reagent tube
Qimin You, Hangzhou (CN); Lin Hu, Hangzhou (CN); Chen Qi, Hangzhou (CN); Junwei Yu, Hangzhou (CN); Zhujun Yu, Hangzhou (CN); Sha Wang, Hangzhou (CN); Rongyu Jin, Hangzhou (CN); Daisang Wang, Hangzhou (CN); Sisi Chen, Hangzhou (CN); Junli He, Hangzhou (CN); Jing Chen, Hangzhou (CN); Huanxin Rao, Hangzhou (CN); Yanqiong Zhou, Hangzhou (CN); and Fan Yang, Hangzhou (CN)
Assigned to USTAR Biotechnologies (Hangzhou) Ltd., Hangzhou (CN)
Filed by USTAR Biotechnologies (Hangzhou) Ltd., Hangzhou (CN)
Filed on Dec. 30, 2021, as Appl. No. 17/646,604.
Application 17/646,604 is a continuation in part of application No. 17/044,734, granted, now 11,242,552, previously published as PCT/CN2019/074312, filed on Feb. 1, 2019.
Claims priority of application No. 201810669579.9 (CN), filed on Jun. 26, 2018.
Prior Publication US 2022/0126286 A1, Apr. 28, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/5082 (2013.01) [B01L 2200/0647 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/16 (2013.01); B01L 2300/161 (2013.01); B01L 2400/0475 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A detection reagent tube for realizing a nucleic acid integrated detection method, the detection reagent tube comprising:
a main tube and one or more branch tubes provided at an end of the main tube, wherein a lysing zone, a cleaning zone and a plurality of separation plugs comprising at least a least a first separation plug and a second separation plug which are sequentially disposed in the main tube, wherein the first separation plug is used for separating the lysing zone and the cleaning zone, a reaction zone is provided in the one or more branch tubes and the second separation plug is positioned at a connection between the one or more branch tubes and the main tube or inside the one or more branch tubes;
hydrophobic layers in a liquid or a solid phase disposed at each of the plurality of separation plugs; and
a magnetic bead channel for magnetically carrying the nucleic acid to sequentially pass through each hydrophobic layer penetrating to the one or more branch tubes defined in an inner wall of the detection reagent tube.