	US 12,465,910 B2
	Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
Vishal Sharma, Hercules, CA (US); Ronald Lebofsky, Hercules, CA (US); Man Cheng, Hercules, CA (US); Adam McCoy, Hercules, CA (US); Kalyan Handique, Hercules, CA (US); and Eric Pomaranski, Hercules, CA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Dec. 7, 2022, as Appl. No. 18/076,667.
Claims priority of provisional application 63/288,405, filed on Dec. 10, 2021.
Prior Publication US 2023/0182127 A1, Jun. 15, 2023
Int. Cl. C12N 13/00 (2006.01); B01L 3/00 (2006.01)

CPC B01L 3/502 (2013.01) [C12N 13/00 (2013.01); B01L 2200/026 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/047 (2013.01); B01L 2300/0893 (2013.01)]

20 Claims

1. A method comprising:

distributing a set of functionalized particles, in a first morphological state, across a set of partitions using a first medium;

transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state;

inducing interactions between the set of functionalized particles and a set of targets of a sample, within the set of partitions and according to a set of operations with a set of process fluids; and

transitioning the set of functionalized particles, at the set of partitions, to a third morphological state with a second medium, contemporaneously with said induced interactions.