US 12,465,875 B2
Process for preparing extracellular vesicles
Aaron Noyes, Melrose, MA (US); Michael Doherty, Somerville, MA (US); Kimberly Ellis, Somerville, MA (US); Raymond Bourdeau, Watertown, MA (US); and Kayla Desanty, Bedford, MA (US)
Assigned to LONZA SALES AG, Basel (CH)
Filed by LONZA SALES AG, Basel (CH)
Filed on Oct. 26, 2022, as Appl. No. 18/050,011.
Application 18/050,011 is a division of application No. 17/593,613, previously published as PCT/US2020/024038, filed on Mar. 20, 2020.
Claims priority of provisional application 62/984,141, filed on Mar. 2, 2020.
Claims priority of provisional application 62/946,895, filed on Dec. 11, 2019.
Claims priority of provisional application 62/903,524, filed on Sep. 20, 2019.
Claims priority of provisional application 62/835,436, filed on Apr. 17, 2019.
Claims priority of provisional application 62/822,013, filed on Mar. 21, 2019.
Prior Publication US 2024/0066433 A1, Feb. 29, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 15/36 (2006.01); A61K 39/00 (2006.01); C12M 1/00 (2006.01)
CPC B01D 15/362 (2013.01) [A61K 39/00 (2013.01); B01D 15/363 (2013.01); C12M 29/10 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of preparing purified extracellular vesicles (EVs) from a sample comprising EVs by a multistep process comprising: (i) contacting the sample with a cation exchange chromatography (CEX) resin (“CEX-process”), thereby obtaining a CEX-processed sample, (ii) contacting the CEX-processed sample with an anion exchange chromatography (AEX) resin (“AEX-process”), thereby obtaining an AEX-processed sample, and (iii) contacting the AEX-processed sample with a mixed-mode chromatography (MMC) resin (“MMC-process”), thereby obtaining an MMC-processed sample,
wherein the sample is contacted with a nuclease, thereby obtaining a nuclease-treated sample, prior to the CEX process, the AEX process, or both.