US 12,465,670 B2
Disposable system and method for preparing a compressed hydrogel
Claude Nicolas Holenstein, Zurich (CH); Jerry Donnan, Edinburgh (GB); and Vincent Ronfard, Villarzel (CH)
Assigned to Cutiss AG, Schlieren (CH)
Filed by Cutiss AG, Schlieren (CH)
Filed on Jan. 17, 2025, as Appl. No. 19/026,572.
Application 19/026,572 is a continuation of application No. 17/907,989, previously published as PCT/IL2021/050225, filed on Mar. 1, 2021.
Claims priority of provisional application 62/983,791, filed on Mar. 2, 2020.
Prior Publication US 2025/0152782 A1, May 15, 2025
Int. Cl. A61L 27/52 (2006.01); A61L 27/24 (2006.01); A61L 27/38 (2006.01); A61L 27/60 (2006.01)
CPC A61L 27/52 (2013.01) [A61L 27/24 (2013.01); A61L 27/3813 (2013.01); A61L 27/60 (2013.01)] 14 Claims
 
1. A disposable system for preparing a compressed hydrogel, the system comprising:
(a) a hydrogel receptacle assembly, comprising:
(i) a graft frame (300) configured to accommodate a hydrogel layer (H); the graft frame (300) comprising a circumferential frame member (301) and a permeable membrane bottom (302) connected to said frame member (301);
(ii) a porous filter plate (200); and
(iii) a base frame (100), comprising a bottom wall (101); a circumferential wall (102) extending from the bottom wall (101), so that the base frame (100) forms a container that is configured to receive the filter plate (200) and the graft frame (300) in an operating mode, such that the filter plate (200) is arranged between the bottom wall (101) of the base frame (100) and the membrane bottom (302) of the graft frame (300); and a plurality of indentations (103) located at one or more edges of the base frame (100);
(b) a removable lid (400); and
(c) a piston assembly comprising:
(i) a piston frame (600), comprising a frame member (601); a support structure (602) extending from a first side (601a) of the frame member (601) towards the membrane bottom (302) in the operating mode; and a plurality of guiding extensions (603) protruding from an edge of the frame member (601) in the compression direction (C); the piston frame (600) is configured to be slidably arranged on the base frame (100) in the operating mode, such that each extension (603) is associated to one of the indentations (103), so that the piston frame (600) is guided by said extensions (603) onto the base frame (100) along the compression direction (C); and
(ii) a piston (500) configured to be connected to the piston frame (600) in a releasable manner, such that in the operating mode the piston frame (600) is configured to press the piston (500) along the compression direction (C) against the hydrogel layer (H) residing in the graft frame (300) so as to compress the hydrogel layer (H) between the piston (500) and the membrane bottom (302) of the graft frame (300);
wherein the graft frame (300), the base frame (100), the filter plate (200), the lid (400), the piston (500) and the piston frame (600) are disposable.