US 12,465,623 B2
Use of parasites and extracellular vesicles obtained from parasites in cancer treatment
Fikrettin Sahin, Istanbul (TR); Zeynep Islek, Istanbul (TR); Ezgi Taskan, Istanbul (TR); Pakize Neslihan Tasli, Istanbul (TR); Batuhan Turhan Bozkurt, Istanbul (TR); Oguz Kaan Kirbas, Istanbul (TR); and Mehmet Hikmet Ucisik, Istanbul (TR)
Assigned to YEDITEPE UNIVERSITESI, Istanbul (TR)
Appl. No. 17/609,795
Filed by YEDITEPE UNIVERSITESI, Istanbul (TR)
PCT Filed May 8, 2020, PCT No. PCT/TR2020/050406
§ 371(c)(1), (2) Date Nov. 9, 2021,
PCT Pub. No. WO2020/231372, PCT Pub. Date Nov. 19, 2020.
Claims priority of application No. 2019/07085 (TR), filed on May 10, 2019.
Prior Publication US 2022/0226393 A1, Jul. 21, 2022
Int. Cl. A61K 39/00 (2006.01); A61K 35/68 (2006.01); A61K 39/008 (2006.01); A61K 39/39 (2006.01); A61P 35/00 (2006.01)
CPC A61K 35/68 (2013.01) [A61K 39/008 (2013.01); A61K 39/39 (2013.01); A61P 35/00 (2018.01); A61K 2039/55588 (2013.01)] 10 Claims
 
1. A pharmaceutical composition for cancer treatment, wherein the composition comprises extracellular vesicles isolated from Leishmania infantum, wherein the extracellular vesicles are isolated from the Leishmania infantum via two-phase liquid system comprising following steps:
collecting a culture media of the Leishmania infantum parasite, wherein the extracellular vesicles are isolated from the parasite,
centrifuging at a rate of 2,000 g to 10,000 g for 5-20 minutes for a removal of undesirable substances such as cell residues and the parasite from the culture media,
removing particles of size 220 nm and above by a filtration after a centrifugation,
transferring a vesicle-protein mixture obtained by the centrifugation into the ATPS containing a PEG phase and a DEX phase for separation f the vesicle-protein mixture,
removing non-vesicular proteins, a cello fat and other impurities from the extracellular vesicles by utilizing a chemical tendency of the PEG phase to the non-vesicular proteins and the DEX phase to phospholipid structured membranes,
obtaining isolated extracellular vesicles.