US 12,465,622 B2
Method of manufacturing diabetic foot patient-specific dermal regeneration sheet and diabetic foot patient-specific dermal regeneration sheet manufactured using the same
Seok Hwan You, Seoul (KR)
Assigned to ROKIT HEALTHCARE INC., Seoul (KR)
Appl. No. 16/630,684
Filed by ROKIT HEALTHCARE INC., Seoul (KR)
PCT Filed Oct. 22, 2019, PCT No. PCT/KR2019/013856
§ 371(c)(1), (2) Date Jan. 13, 2020,
PCT Pub. No. WO2021/071001, PCT Pub. Date Apr. 15, 2021.
Claims priority of application No. 10-2019-0124693 (KR), filed on Oct. 8, 2019.
Prior Publication US 2021/0401897 A1, Dec. 30, 2021
Int. Cl. A61K 35/35 (2015.01); A61K 9/00 (2006.01); A61K 9/70 (2006.01); A61K 35/15 (2025.01); A61K 35/17 (2025.01); A61K 35/28 (2015.01); A61K 35/36 (2015.01); A61K 35/44 (2015.01); A61K 38/20 (2006.01); A61K 38/22 (2006.01); A61K 38/28 (2006.01); A61K 38/30 (2006.01); A61K 38/36 (2006.01); A61K 38/48 (2006.01); A61P 3/10 (2006.01); A61P 17/02 (2006.01); C07K 14/52 (2006.01); C07K 14/54 (2006.01); C07K 14/78 (2006.01); C12N 5/077 (2010.01); C07K 14/81 (2006.01)
CPC A61K 35/35 (2013.01) [A61K 9/0014 (2013.01); A61K 9/7007 (2013.01); A61K 35/15 (2013.01); A61K 35/17 (2013.01); A61K 35/28 (2013.01); A61K 35/36 (2013.01); A61K 35/44 (2013.01); A61K 38/2006 (2013.01); A61K 38/204 (2013.01); A61K 38/2264 (2013.01); A61K 38/28 (2013.01); A61K 38/30 (2013.01); A61K 38/363 (2013.01); A61K 38/4833 (2013.01); A61P 3/10 (2018.01); A61P 17/02 (2018.01); C12N 5/0653 (2013.01); C07K 14/521 (2013.01); C07K 14/54 (2013.01); C07K 14/78 (2013.01); C07K 14/8125 (2013.01); C07K 2299/00 (2013.01)] 9 Claims
 
1. A method of manufacturing a diabetic foot patient-specific skin regeneration sheet, comprising:
A) extracting autologous adipose tissue;
B) removing fibers in the autologous adipose tissue using a first filter having a pore diameter of 2 to 3 mm, retaining the fibers on the first filter and passing the autologous adipose tissue through the first filter;
C) crushing the autologous adipose tissue from which the fibers are removed by sequentially passing it through a second filter having a pore diameter of 450 to 550 μm and a third filter having a pore diameter of 150 to 250 μm, producing a crushed autologous adipose tissue;
D) acquiring an adipose tissue extract by filtrating the crushed autologous adipose tissue mixed with a saline solution by a fourth filter having a pore diameter of 25 to 75 μm, acquiring a material collected by the fourth filter to be used as adipose tissue extract, and disposing of the filtrate;
E) obtaining 3D data of a skin defect area of a patient using a 3D scanner, and then based on the data, manufacturing a 3D mold corresponding to the skin defect area using a 3D printer;
F) forming a proto-layer by applying a first solution containing the adipose tissue extract and fibrinogen to the inside of the 3D mold;
G) curing the proto-layer into a skin regeneration layer by applying a second solution containing thrombin onto the proto-layer; and
H) removing the 3D mold;
wherein the volume ratio of the adipose tissue extract and the fibrinogen in the first solution is 3:1 to 5:1.