	US 12,465,573 B2
	Structured nanocoatings for the stabilization of pluripotent stem cell media components
William L. Murphy, Waunakee, WI (US); Andrew Khalil, Madison, WI (US); Angela Xie, Middleton, WI (US); and Hunter Johnson, Brooklyn, WI (US)
Assigned to WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Appl. No. 17/045,293
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
PCT Filed Apr. 5, 2019, PCT No. PCT/US2019/026086 § 371(c)(1), (2) Date Oct. 5, 2020, PCT Pub. No. WO2019/195748, PCT Pub. Date Oct. 10, 2019.
Claims priority of provisional application 62/653,847, filed on Apr. 6, 2018.
Prior Publication US 2021/0145757 A1, May 20, 2021
Int. Cl. A61K 9/50 (2006.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01)

CPC A61K 9/501 (2013.01) [C12N 5/0606 (2013.01); C12N 5/0696 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2531/00 (2013.01); C12N 2533/18 (2013.01); C12N 2533/90 (2013.01)]

8 Claims

1. A pluripotent stem cell culture method for maintaining stem cell pluripotency, the method comprising: contacting a pluripotent stem cell with a mineral coated microparticle, wherein the mineral coated microparticle comprises a core and a mineral coating that comprises fibroblast growth factor in an amount of fibroblast growth factor ranging from about 0.1 ng fibroblast growth factor per mg mineral coated microparticle to about 10,000 ng fibroblast growth factor per mg mineral coated microparticle, and wherein stem cell pluripotency is maintained when a minimum of 95% Oct4⁺/Nanog⁺ cell population is maintained at each cell passage.